With the increasing use of nanomaterials in recent years, determining their comparative toxicities has become a subject of intense research interest. However, the variety of test methods available for each material makes it difficult to compare toxicities. Here, an accurate and reliable method is developed to evaluate the toxicity of manufactured nanomaterials, such as Al2O3, carbon black, single-walled carbon nanotubes (SWCNTs), multi-walled carbon nanotubes (MWCNTs), CeO2, dendrimers, fullerene, gold, iron, nanoclays, silver, SiO2, TiO2, and ZnO. A series of 72 h chronic and 8 h acute toxicity tests was performed using cell counting, chlorophyll, and delayed fluorescence methods. Comparable toxicities using the chlorophyll and delayed fluorescence methods were impossible to determine because the EC50 of some of the nanomaterials could not be measured. All three test methods were successfully applied to the chronic toxicity tests of manufactured nanomaterials, and cell counting was the only method applicable to acute toxicity tests. The toxicity data and the proposal of measurement method for manufactured nanomaterials obtained in this study can be helpful for preparing exposure standards and investigating the toxicities of other nanomaterials in the future.
Keywords: Raphidocelis subcapitata; cell count method; chlorophyll fluorescence method; delayed fluorescence method; ecotoxicity; manufactured nanomaterial.