Using Vertebrate Stem and Progenitor Cells for Cellular Agriculture, State-of-the-Art, Challenges, and Future Perspectives

Teodora Knežić; Ljiljana Janjušević; Mila Djisalov; Supansa Yodmuang; Ivana Gadjanski

doi:10.3390/biom12050699

Using Vertebrate Stem and Progenitor Cells for Cellular Agriculture, State-of-the-Art, Challenges, and Future Perspectives

Biomolecules. 2022 May 13;12(5):699. doi: 10.3390/biom12050699.

Authors

Teodora Knežić¹, Ljiljana Janjušević¹, Mila Djisalov¹, Supansa Yodmuang², Ivana Gadjanski¹

Affiliations

¹ Center for Biosystems, BioSense Institute, University of Novi Sad, Dr. Zorana Djindjica 1, 21000 Novi Sad, Serbia.
² Research Affairs, Faculty of Medicine, Chulalongkorn University, 1873 Rama 4 Rd, Pathumwan, Bangkok 10330, Thailand.

Abstract

Global food systems are under significant pressure to provide enough food, particularly protein-rich foods whose demand is on the rise in times of crisis and inflation, as presently existing due to post-COVID-19 pandemic effects and ongoing conflict in Ukraine and resulting in looming food insecurity, according to FAO. Cultivated meat (CM) and cultivated seafood (CS) are protein-rich alternatives for traditional meat and fish that are obtained via cellular agriculture (CA) i.e., tissue engineering for food applications. Stem and progenitor cells are the building blocks and starting point for any CA bioprocess. This review presents CA-relevant vertebrate cell types and procedures needed for their myogenic and adipogenic differentiation since muscle and fat tissue are the primary target tissues for CM/CS production. The review also describes existing challenges, such as a need for immortalized cell lines, or physical and biochemical parameters needed for enhanced meat/fat culture efficiency and ways to address them.

Keywords: cellular agriculture; cultured meat; cultured seafood; progenitor cells; stem cells; tissue engineering.

Publication types

Review
Research Support, Non-U.S. Gov't

MeSH terms

Agriculture
Animals
COVID-19*
Fishes
Humans
Meat
Pandemics*
Stem Cells

Grants and funding

This research was funded by REALSENSE1: Monitoring of cell culture parameters using sensors for biomass and nutrients/metabolites in media: Lab-on-a-Chip (LOC) approach, Good Food Institute 2018 Competitive Grant Program and REALSENSE2: From Lab-On-A-Chip to Custom Bioreactor: Scale Up Modeling Study, Good Food Institute 2019 Competitive Grant Program; DAAD-Ministry of Education, Science and Technological Development of the Republic of Serbia bilateral collaboration project between Biosense Institute and Fraunhofer Institute for Process Engineering and Packaging IVV titled: Development of plant-based edible microcarriers and scaffolds for use in cellular agriculture i.e., cultivated meat tissue engineering (2021–2022) and MSCA-RISE H2020 project IPANEMA grant agreement N° 872662. I.G. participates in a project that has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement N° 739570 (ANTARES). The financial support of the Ministry of Education, Science and Technological Development of the Republic of Serbia is acknowledged—Grant N° 451-03-68/2022-14/200358 for authors T.K., M.D. and L.J. S.Y. has received Program Manage Unit Competitiveness (PMUC)—Grant N° C10F64005.