2-Hydroxy-4-Methylselenobutanoic Acid Promotes Follicle Development by Antioxidant Pathway

Shengyu Xu; Yanpeng Dong; Sirun Chen; Yalei Liu; Zimei Li; Xinlin Jia; Mickael Briens; Xuemei Jiang; Yan Lin; Lianqiang Che; Yong Zhuo; Jian Li; Bin Feng; Zhengfeng Fang; Jianping Wang; Zhihua Ren; De Wu

doi:10.3389/fnut.2022.900789

2-Hydroxy-4-Methylselenobutanoic Acid Promotes Follicle Development by Antioxidant Pathway

Front Nutr. 2022 May 10:9:900789. doi: 10.3389/fnut.2022.900789. eCollection 2022.

Authors

Shengyu Xu¹, Yanpeng Dong¹, Sirun Chen¹, Yalei Liu¹, Zimei Li¹, Xinlin Jia¹, Mickael Briens², Xuemei Jiang¹, Yan Lin¹, Lianqiang Che¹, Yong Zhuo¹, Jian Li¹, Bin Feng¹, Zhengfeng Fang¹, Jianping Wang¹, Zhihua Ren³, De Wu¹

Affiliations

¹ Key Laboratory of Animal Disease-Resistant Nutrition, Ministry of Education, Ministry of Agriculture and Rural Affairs, Key Laboratory of Sichuan Province, Animal Nutrition Institute, Sichuan Agricultural University, Chengdu, China.
² Adisseo France SAS, Commentry, France.
³ Sichuan Province Key Laboratory of Animal Disease and Human Health, Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.

Abstract

Selenium (Se) is assumed to promote the follicle development by attenuating oxidative stress. The current study was developed to evaluate the effects of dietary 2-hydroxy-4-methylselenobutanoic acid (HMSeBA) supplementation on the follicle development in vivo and on the function of ovarian granulosa cells (GCs) in vitro. Thirty-six gilts were randomly assigned to fed control diet (CON), Na₂SeO₃ diet (0.3 mg Se/kg) or HMSeBA diet (0.3 mg Se/kg). The results showed that HMSeBA and Na₂SeO₃ supplementation both increased the total selenium content in liver and serum compared with control, while HMSeBA increased the total selenium content in liver compared with Na₂SeO₃ group. HMSeBA tended to increase the total selenium content in ovary compared with control. HMSeBA and Na₂SeO₃ supplementation both increased the weight of uteri in gilts at the third estrus. Moreover, HMSeBA supplementation down-regulated the gene expression of growth differentiation factor-9 (GDF-9) and bone morpho-genetic protein-15 (BMP-15) in cumulus-oocyte complexes (COCs). HMSeBA supplementation decreased malondialdehyde (MDA) content in serum, liver and ovary, increased activity of T-AOC in liver, TXNRD in ovary and GPX in serum, liver and ovary, while up-regulated the liver GPX2, SOD1 and TXNRD1, ovarian GPX1 gene expression. In vitro, HMSeBA treatment promoted GCs' proliferation and secretion of estradiol (E₂). HMSeBA treatment increased the activity of T-AOC, T-SOD, GPX, TXNRD and decreased MDA content in GCs in vitro. Meanwhile, HMSeBA treatment up-regulated SOD2 and GPX1 gene expression in GCs in vitro. In conclusion, HMSeBA supplementation is more conducive to promoting follicle development by antioxidant pathway.

Keywords: 2-hydroxy-4-methylselenobutanoic acid (HMSeBA); antioxidant capacity; follicle development; gilt; granulose cell.