Over the recent years, mass spectrometry (MS)-based proteomics has undergone dramatic advances in sample preparation, instrumentation, and computational methods. Here, we describe in detail, how a workflow quantifies global protein phosphorylation in pancreatic islets and characterizes intracellular organelle composition on protein level by MS-based proteomics.
Keywords: Mass spectrometry-based phosphoproteomics; Organelles; Pancreatic islets; Protein correlation profiling.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.