Characterization of Bioactive Sialyl Oligosaccharides Separated from Colostrum of Indonesia Dairy Goat

Epi Taufik; Irma Isnafia Arief; Cahyo Budiman; Yusuf Wibisono; Alfian Noviyanto

doi:10.5851/kosfa.2022.e13

Characterization of Bioactive Sialyl Oligosaccharides Separated from Colostrum of Indonesia Dairy Goat

Food Sci Anim Resour. 2022 May;42(3):426-440. doi: 10.5851/kosfa.2022.e13. Epub 2022 May 1.

Authors

Epi Taufik¹, Irma Isnafia Arief¹, Cahyo Budiman¹, Yusuf Wibisono², Alfian Noviyanto^{3

4}

Affiliations

¹ Department of Animal Production and Technology, Faculty of Animal Science, IPB University, Bogor 16680, Indonesia.
² Department of Bioprocess Engineering, Brawijaya University, Malang 65145, Indonesia.
³ Department of Mechanical Engineering, Mercu Buana University, Jakarta 11650, Indonesia.
⁴ Nano Center Indonesia, Banten 15314, Indonesia.

Abstract

The bioactive functions of oligosaccharides from human milk have been reported by many studies. Many of oligosaccharides isolated from colostrum and/or milk of dairy animals have been reported to have similar chemical structures with those in human colostrum and/or milk. It has been proved by several studies that the oligosaccharides with similar chemical structure shared common bioactivities. Among domesticated dairy animals, bovine/cattle, caprine/goat, and ovine/sheep are the most commonly used species to isolate oligosaccharides from their colostrum and/or milk. Several studies on the oligosaccharides from goat colostrum and milk have revealed similar properties to that of human milk and possess the highest content of sialyl oligosaccharides (SOS) as compared to other ruminants. Indonesia ranks first in Association of Southeast Asian Nations (ASEAN) for goat milk production. Therefore, goat milk is the second most consumed milk in the country. The most reared dairy goat breed in Indonesia is Etawah Grade. However, oligosaccharides from Indonesia dairy animals including goat, have not been characterized. This is the first study to characterize oligosaccharides from Indonesia dairy animals. The present study was aimed to isolate and characterize oligosaccharides, specifically SOS from the colostrum of Etawah Grade goats by using proton/¹H-nuclear magnetic resonance. The SOS successfully characterized in this study were: Neu5Ac(α2-3)Gal(β1-4)Glc (3'-N-acetylneuraminyllactose), Neu5Ac(α2-6)Gal(β1-4)Glc (6'-N-acetylneuraminyllactose), Neu5Gc(α2-3)Gal(β1-4)Glc (3'-N-glycolylneuraminyllactose), Neu5Gc(α2-6)Gal(β1-4)Glc (6'-N-glycolylneuraminyllactose), Neu5Ac(α2-6)Gal(β1-4) GlcNAc (6'-N-acetylneuraminyllactosamine) and Neu5Gc(α2-6)Gal(β1-4)GlcNAc (6'-N-glycolylneuraminyllactosamine). This finding shows that Etawah Grade, as a local dairy goat breed in Indonesia, is having significant potential to be natural source of oligosaccharides that can be utilized in the future food and pharmaceutical industries.

Keywords: Etawah Grade; colostrum; dairy goat; milk; oligosaccharides.