Alkaline deacetylation-aided hydrogen peroxide-acetic acid pretreatment of bamboo residue to improve enzymatic saccharification and bioethanol production

Fanyang Meng; Haiyan Yang; Zhengjun Shi; Ping Zhao; Jing Yang

doi:10.1016/j.biortech.2022.127321

Alkaline deacetylation-aided hydrogen peroxide-acetic acid pretreatment of bamboo residue to improve enzymatic saccharification and bioethanol production

Bioresour Technol. 2022 Aug:358:127321. doi: 10.1016/j.biortech.2022.127321. Epub 2022 May 21.

Authors

Fanyang Meng¹, Haiyan Yang², Zhengjun Shi², Ping Zhao¹, Jing Yang³

Affiliations

¹ School of Chemical Engineering, Southwest Forestry University, Kunming 650224, China.
² Key Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of China, Ministry of Education, Southwest Forestry University, Kunming 650224, China; School of Chemical Engineering, Southwest Forestry University, Kunming 650224, China.
³ Key Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of China, Ministry of Education, Southwest Forestry University, Kunming 650224, China; School of Chemical Engineering, Southwest Forestry University, Kunming 650224, China. Electronic address: kmjingyang@163.com.

PMID: 35609748
DOI: 10.1016/j.biortech.2022.127321

Abstract

Bamboo pretreatment with alkaline deacetylation-aided hydrogen peroxide-acetic acid (HPAC-NaOH) was investigated for producing high-value-added products. Comparing with HPAC pretreated D. sinicus, the post-treatment of alkaline deacetylation resulted in higher glucose yield of 91.3% and ethanol concentrations of 17.20 g/L, increased by about 20-27%. A strong negative correlation between the content of acetyl with cellulose accessibility and enzymatic hydrolysis yield was showed. The deacetylation of HPAC-DS contributed to the increase of cellulase adsorption capacities in substrates and the variations of hydrophilicity, cellulose crystallinity, and degree of polymerization, which can generate highly reactive cellulosic materials for enzymatic saccharification to produce bioethanol. The HPAC-NaOH pretreatment can provide a promising approach to improve the bioconversion of bamboo to biofuels, and has broad space for the biorefinery of bamboo in the south of China.

Keywords: Bamboo residues; Cellulose accessibility; Delignification; Enzymatic hydrolysis; Hydrogen peroxide-acetic acid (HPAC) pretreatment.

MeSH terms

Acetic Acid*
Cellulase* / chemistry
Cellulose / chemistry
Hydrogen Peroxide
Hydrolysis
Lignin / chemistry
Sodium Hydroxide

Substances

Sodium Hydroxide
Cellulose
Lignin
Hydrogen Peroxide
Cellulase
Acetic Acid