Oxysterols profiles in zebrafish (Danio rerio) embryos exposed to bisphenol A

Anton Vremere; Carmine Merola; Federico Fanti; Manuel Sergi; Monia Perugini; Dario Compagnone; Milena Mikhail; Stefano Lorenzetti; Michele Amorena

doi:10.1016/j.fct.2022.113166

Oxysterols profiles in zebrafish (Danio rerio) embryos exposed to bisphenol A

Food Chem Toxicol. 2022 Jul:165:113166. doi: 10.1016/j.fct.2022.113166. Epub 2022 May 21.

Authors

Anton Vremere¹, Carmine Merola², Federico Fanti³, Manuel Sergi⁴, Monia Perugini⁵, Dario Compagnone⁶, Milena Mikhail⁷, Stefano Lorenzetti⁸, Michele Amorena⁹

Affiliations

¹ Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Italy; Dpt. of Food Safety, Nutrition and Veterinary Public Health, Istituto Superiore di Sanità - ISS, Rome, Italy. Electronic address: avremere@unite.it.
² Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Italy. Electronic address: cmerola@unite.it.
³ Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Italy. Electronic address: ffanti@unite.it.
⁴ Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Italy. Electronic address: msergi@unite.it.
⁵ Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Italy. Electronic address: mperugini@unite.it.
⁶ Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Italy. Electronic address: dcompagnone@unite.it.
⁷ Dpt. of Food Safety, Nutrition and Veterinary Public Health, Istituto Superiore di Sanità - ISS, Rome, Italy. Electronic address: milena.mikhail@iss.it.
⁸ Dpt. of Food Safety, Nutrition and Veterinary Public Health, Istituto Superiore di Sanità - ISS, Rome, Italy. Electronic address: stefano.lorenzetti@iss.it.
⁹ Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Italy. Electronic address: mamorena@unite.it.

PMID: 35609738
DOI: 10.1016/j.fct.2022.113166

Abstract

Introduction: Oxysterols are cholesterol oxidation products and bioactive lipids involved in developmental signalling pathways, embryonic and postembryonic tissue patterning and homeostasis. The embryonic period is a very sensitive window of exposure to bisphenol A (BPA), hence the role of BPA on the levels of oxysterols in the very early stages of zebrafish embryogenesis is a relevant novel field of investigation.

Objectives: To compare the role of BPA on oxysterols levels in zebrafish embryos at 8 and 24 h post fertilization (hpf) with cytochromes P450 (CYPs)-modulating chemicals (carbamazepine, ketoconazole, and hydrogen peroxide).

Methods: Upon a dose range finding, zebrafish embryos were exposed to environmentally relevant (0.04 μM) and toxicological (17.5 μM) BPA concentrations. Seven oxysterols were profiled by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS).

Results: Similarly to the CYPs-modulating chemicals, BPA caused: i) no significant changes at 8 hpf and ii) a dose-dependent increase of total oxysterols at 24 hpf, with 27-hydroxycholesterol as the most regulated oxysterol.

Discussion: In the first day post-fertilization of the zebrafish embryos, the role of BPA alike a CYPs-modulating chemical was confirmed by the similar oxysterol changes observed with the already known CYPs-modulating chemicals.

Keywords: Bisphenol A; Carbamazepine; Hydrogen peroxide; Ketoconazole; Oxysterols; Zebrafish embryos.

MeSH terms

Animals
Benzhydryl Compounds / metabolism
Benzhydryl Compounds / toxicity
Embryo, Nonmammalian / metabolism
Oxysterols* / metabolism
Phenols
Tandem Mass Spectrometry
Water Pollutants, Chemical* / metabolism
Zebrafish / metabolism

Substances

Benzhydryl Compounds
Oxysterols
Phenols
Water Pollutants, Chemical
bisphenol A