Antagonistic activity and mode of action of trypacidin from marine-derived Aspergillus fumigatus against Vibrio parahaemolyticus

Siya Guo; Zongyi Zhang; Xiaowen Xu; Jing Cai; Wenbin Wang; Lei Guo

doi:10.1007/s13205-022-03194-3

Antagonistic activity and mode of action of trypacidin from marine-derived Aspergillus fumigatus against Vibrio parahaemolyticus

3 Biotech. 2022 Jun;12(6):131. doi: 10.1007/s13205-022-03194-3. Epub 2022 May 20.

Authors

Siya Guo^{1

2

3}, Zongyi Zhang^{1

2}, Xiaowen Xu^{1

2

3}, Jing Cai^{1

2}, Wenbin Wang^{1

2}, Lei Guo^{1

2

3}

Affiliations

¹ Jiangsu Key Laboratory of Marine Bioresources and Environment, Co-Innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Ocean University, Lianyungang, 222005 China.
² Jiangsu Key Laboratory of Marine Biotechnology, School of Food Science and Engineering, Jiangsu Ocean University, Lianyungang, 222005 China.
³ Jiangsu Key Laboratory of Marine Pharmaceutical Compound Screening, School of Pharmacy, Jiangsu Ocean University, Lianyungang, 222005 China.

Abstract

This study aimed to investigate the antagonistic activity and mode of action of trypacidin from marine-derived Aspergillus fumigatus against Vibrio parahaemolyticus. Results indicated that the minimal inhibitory concentration and minimal bactericidal concentration of trypacidin against V. parahaemolyticus were 31.25 and 62.5 μg/mL, respectively, which was better than that of streptomycin sulfate. Trypacidin remarkably inhibited the growth of V. parahaemolyticus and had a strong destructive effect on cell wall permeability and integrity, cell membrane permeability, and morphological alterations. Its potential as an antibacterial agent for aquatic products must be further explored.

Keywords: Antagonistic activity; Aquatic pathogen; Mode of action; Trypacidin; Vibrio parahaemolyticus.