Electrochemiluminescence aptasensor for vascular endothelial growth factor 165 detection based on Ru(bpy)32+/Au nanoparticles film modified electrode and double signal amplification

Cuiying Lin; Qingqing Huang; Xiang Hong; Shichai Hong; Xiaolong Shu; Enci Wang; Lixin Wang; Weiguo Fu; Zhenyu Lin

doi:10.1016/j.bioelechem.2022.108151

Electrochemiluminescence aptasensor for vascular endothelial growth factor 165 detection based on Ru(bpy)₃²⁺/Au nanoparticles film modified electrode and double signal amplification

Bioelectrochemistry. 2022 Aug:146:108151. doi: 10.1016/j.bioelechem.2022.108151. Epub 2022 May 13.

Authors

Cuiying Lin¹, Qingqing Huang¹, Xiang Hong², Shichai Hong², Xiaolong Shu³, Enci Wang³, Lixin Wang⁴, Weiguo Fu⁵, Zhenyu Lin⁶

Affiliations

¹ Ministry of Education Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian 350116, China.
² Department of Vascular Surgery, Zhongshan Xiamen Hospital, Fudan University, 668 JinhuRoad, Xiamen 361015, China.
³ Department of Vascular Surgery, Zhongshan Hospital, Fudan University, 180 Fenglin Road, Shanghai 200032, China.
⁴ Department of Vascular Surgery, Zhongshan Xiamen Hospital, Fudan University, 668 JinhuRoad, Xiamen 361015, China; Department of Vascular Surgery, Zhongshan Hospital, Fudan University, 180 Fenglin Road, Shanghai 200032, China. Electronic address: wang.lixin@zs-hospital.sh.cn.
⁵ Department of Vascular Surgery, Zhongshan Xiamen Hospital, Fudan University, 668 JinhuRoad, Xiamen 361015, China; Department of Vascular Surgery, Zhongshan Hospital, Fudan University, 180 Fenglin Road, Shanghai 200032, China. Electronic address: WeiguoFu@zs-hospital.sh.cn.
⁶ Ministry of Education Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian 350116, China. Electronic address: zylin@fzu.edu.cn.

PMID: 35605452
DOI: 10.1016/j.bioelechem.2022.108151

Abstract

Vascular endothelial growth factor (VEGF₁₆₅) is a signal protein that plays a central role in the regulation of angiogenesis and can stimulate angiogenesis. The development of highly sensitive and selective detection method for VEGF₁₆₅ is very important for disease diagnosis and follow-up treatment monitoring. In this study, an electrochemiluminescence (ECL) aptasensor for VEGF₁₆₅ has been developed based on quench of H₂O₂ toward Ru(bpy)₃²⁺/TPrA ECL system and RecJ_f exonuclease induced target recovery and hybridization chain reaction (HCR) as amplification strategy. The presence of VEGF₁₆₅ makes a large number of glucose oxidase (GOD) fixed on the electrode surface through the double signal amplification strategies. The present of GOD cause the production of a large amount of H₂O₂ near the electrode surface under excess amount of glucose, resulting in the inhibition of the ECL signal of Ru(bpy)₃²⁺/Au nanoparticles (Ru(bpy)₃²⁺/AuNPs) film fixed on the electrode surface. The ECL response of the designed biosensor has a good linear relationship with the logarithm of the concentration of VEGF₁₆₅ in the range of 0.5 pg/mL to 500 ng/mL with a detection limit of 0.2 fg/mL. The VEGF₁₆₅ in serum samples has been detected by the proposed aptasensor with satisfactory results.

Keywords: Aptasensors; Electrochemiluminescence; Hybridization chain reaction; RecJ(f) exonuclease; Vascular endothelial growth factor 165.

MeSH terms

2,2'-Dipyridyl
Biosensing Techniques* / methods
Electrochemical Techniques / methods
Electrodes
Glucose Oxidase
Gold
Hydrogen Peroxide
Luminescent Measurements / methods
Metal Nanoparticles*
Ruthenium Compounds
Vascular Endothelial Growth Factor A

Substances

Ruthenium Compounds
Vascular Endothelial Growth Factor A
2,2'-Dipyridyl
Gold
Hydrogen Peroxide
Glucose Oxidase