MNSs-mediated N-glycan processing is essential for auxin homeostasis in Arabidopsis roots during alkaline response

Tianyu Xia; Yujie Zhan; Yangjie Mu; Jianhua Zhang; Weifeng Xu

doi:10.1016/j.isci.2022.104298

MNSs-mediated N-glycan processing is essential for auxin homeostasis in Arabidopsis roots during alkaline response

iScience. 2022 Apr 25;25(5):104298. doi: 10.1016/j.isci.2022.104298. eCollection 2022 May 20.

Authors

Tianyu Xia¹, Yujie Zhan¹, Yangjie Mu¹, Jianhua Zhang², Weifeng Xu¹

Affiliations

¹ Center for Plant Water-use and Nutrition Regulation and College of Life Sciences, Joint International Research Laboratory of Water and Nutrient in Crop and College of Resource and Environment, Fujian Agriculture and Forestry University, Jinshan, Fuzhou 350002, China.
² Department of Biology, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China.

Abstract

Early steps in the endoplasmic reticulum (ER) lumen and cis-Golgi comprise trimming of N-glycans by class I α-mannosidases (MNSs) play crucial roles in root growth and stress response. Herein, we found that the root growth inhibition in the mns1 mns2 mns3 mutant was partially rescued under alkaline condition, and inhibitor treatment to disrupt auxin transport counteracted this alkaline-maintained root growth. Further study showed that indole-3-acetic acid (IAA) levels were undetectable in mns1 mns2 mns3 at normal condition and recovered at alkaline condition, which corroborate our N-glycopeptide profiling, from which N-glycopeptides related with IAA biosynthesis, amino acid conjugates hydrolysis, and response showed differential abundance between normal and alkaline conditions in mns1 mns2 mns3. Overall, our results linked the need for MNSs-mediated N-glycan processing in the ER and cis-Golgi with maintenance of auxin homeostasis and transport in Arabidopsis roots during the response to alkaline stress.

Keywords: Biological sciences; Plant biology; Plant physiology; Plants.