Generation of pigs with humanized type II collagen by precise human COL2A1 gene knock-in

Ting Lan; Yuling Zheng; Yangyang Suo; Yuhui Wei; Hui Shi; Quanmei Yan; Zhenpeng Zhuang; Huangyao Chen; Quanjun Zhang; Nana Fan; Yu Zhao; Zhen Ouyang; Chengdan Lai; Zhaoming Liu; Jizeng Zhou; Chengcheng Tang; Nam-Hyung Kim; Qingjian Zou; Xiaomin Wang

doi:10.1016/j.jgg.2022.05.001

Generation of pigs with humanized type II collagen by precise human COL2A1 gene knock-in

J Genet Genomics. 2023 Mar;50(3):212-215. doi: 10.1016/j.jgg.2022.05.001. Epub 2022 May 20.

Authors

Ting Lan¹, Yuling Zheng², Yangyang Suo³, Yuhui Wei⁴, Hui Shi⁴, Quanmei Yan⁴, Zhenpeng Zhuang⁴, Huangyao Chen⁴, Quanjun Zhang⁴, Nana Fan⁴, Yu Zhao⁴, Zhen Ouyang⁴, Chengdan Lai⁴, Zhaoming Liu⁴, Jizeng Zhou⁵, Chengcheng Tang², Nam-Hyung Kim², Qingjian Zou⁶, Xiaomin Wang⁷

Affiliations

¹ Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Wuyi University, Jiangmen, Guangdong 529020, China; University of Chinese Academy of Sciences, Beijing 100049, China; CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, Guangdong 510530, China.
² Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Wuyi University, Jiangmen, Guangdong 529020, China.
³ Joint School of Life Science, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou Medical University, Guangzhou, Guangdong 510530, China.
⁴ Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Wuyi University, Jiangmen, Guangdong 529020, China; CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, Guangdong 510530, China.
⁵ The School of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou, Guangdong 510006, China.
⁶ Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Wuyi University, Jiangmen, Guangdong 529020, China. Electronic address: zouqj@wyu.edu.cn.
⁷ CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, Guangdong 510530, China; School of Life Sciences, Westlake University, Hangzhou, Zhejiang 310024, China; Institute of Biology, Westlake Institute for Advanced Study, Hangzhou, Zhejiang 310024, China. Electronic address: wangxiaomin85@126.com.

PMID: 35598861
DOI: 10.1016/j.jgg.2022.05.001

No abstract available

Publication types

Letter
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Collagen Type II* / genetics
Gene Knock-In Techniques
Humans
Swine / genetics

Substances

Collagen Type II
COL2A1 protein, human