Localized plasmonic sensor for direct identifying lung and colon cancer from the blood

Chenglong Lin; Shunshun Liang; Yanyan Li; Yusi Peng; Zhengren Huang; Zhiyuan Li; Yong Yang; Xiaoying Luo

doi:10.1016/j.bios.2022.114372

Localized plasmonic sensor for direct identifying lung and colon cancer from the blood

Biosens Bioelectron. 2022 Sep 1:211:114372. doi: 10.1016/j.bios.2022.114372. Epub 2022 May 15.

Authors

Chenglong Lin¹, Shunshun Liang², Yanyan Li¹, Yusi Peng¹, Zhengren Huang³, Zhiyuan Li⁴, Yong Yang⁵, Xiaoying Luo⁶

Affiliations

¹ State Key Laboratory of High-Performance Ceramics and Superfine Microstructures, Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai, 200050, China; Graduate School of the Chinese Academy of Sciences, No.19(A) Yuquan Road, Beijing, 100049, China; Center of Materials Science and Optoelectronics Engineering, University of Chinese Academy of Sciences, Beijing, 100049, China.
² State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji Hospital, Shanghai Jiaotong University School of Medicine, 200032, Shanghai, China.
³ State Key Laboratory of High-Performance Ceramics and Superfine Microstructures, Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai, 200050, China.
⁴ School of Physics and Optoelectronics, South China University of Technology, Guangzhou, 510641, China.
⁵ State Key Laboratory of High-Performance Ceramics and Superfine Microstructures, Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai, 200050, China; Center of Materials Science and Optoelectronics Engineering, University of Chinese Academy of Sciences, Beijing, 100049, China. Electronic address: yangyong@mail.sic.ac.cn.
⁶ State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji Hospital, Shanghai Jiaotong University School of Medicine, 200032, Shanghai, China. Electronic address: luoxy@shsci.org.

PMID: 35598554
DOI: 10.1016/j.bios.2022.114372

Abstract

The tissue inhibitor of metalloproteinases-1 (TIMP-1) protein can regulate the expression of certain proteases and microRNAs in cancer cells, and it is highly possible to diagnose cancers through analyzing the expression of TIMP-1 on exosomes. However, it is still a great challenge to obtain reliable physiological information on TIMP-1 by label-free method from exosomes in plasma. Here, we designed a porous-plasmonic SERS chip functionalized with synthesized CP05 polypeptide, which can specifically capture and distinguish exosomes from diverse origins. The SERS chip can accurately locate the plasmon in TIMP-1 protein to analyze the discrepancy of related fingerprint peaks of different exosomes. Based on the designed SERS chip, we successfully distinguished the lung and colon cancer cell-derived exosomes from normal exosomes at the single vesicle level by unique Raman spectroscopy and machine learning methods. This work not only provides a practical SERS chip for the application of Raman technology in human tumor monitoring and prognosis, but also provides a new idea for analyzing the feature of exosomes at the spectral level.

Keywords: Exosomes; Machine learning; Plasmon; SERS; TIMP-1; Tumor.

MeSH terms

Biosensing Techniques*
Cell Line, Tumor
Colonic Neoplasms* / diagnosis
Exosomes* / chemistry
Humans
Lung
Lung Neoplasms* / metabolism
Spectrum Analysis, Raman / methods
Tissue Inhibitor of Metalloproteinase-1 / analysis
Tissue Inhibitor of Metalloproteinase-1 / metabolism

Substances

Tissue Inhibitor of Metalloproteinase-1