Molecular and morphological characterization of Dollfustrema bagarii (Digenea: Bucephalidae) metacercariae from aquaculture channel catfish (Ictalurus punctatus) in northern Vietnam

J Fish Dis. 2022 Aug;45(8):1165-1171. doi: 10.1111/jfd.13651. Epub 2022 May 21.

Abstract

White grub metacercariae were found in the livers and kidneys of diseased specimens of an introduced channel catfish, Ictalurus punctatus (Rafinesque), in Vietnam. Based on morphological features and 28S rDNA sequence analysis, the isolated metacercariae were identified as Dollfustrema bagarii (Digenea: Bucephalidae) Moravec & Sey. Histopathological examination shows that encysted metacercariae can change the tissue structure of the infected organs and is often accompanied by haemorrhaging and the presence of eosinophilic granular cell infiltration. Degenerative changes were also observed.

Keywords: 28S rDNA; fish disease; histopathological examination; introduced fish; phylogenetic analysis.

MeSH terms

  • Animals
  • Aquaculture
  • Fish Diseases*
  • Ictaluridae*
  • Metacercariae / genetics
  • Trematoda* / genetics
  • Trematode Infections* / veterinary
  • Vietnam