Electrochemical investigation of DNA-metal complex interactions and development of a highly sensitive electrochemical biosensor

Majid Arvand; Hoda Ilkhani; Mohamad Reza Ganjali; Akram Pourhabib

doi:10.1016/j.ab.2022.114738

Electrochemical investigation of DNA-metal complex interactions and development of a highly sensitive electrochemical biosensor

Anal Biochem. 2022 Sep 1:652:114738. doi: 10.1016/j.ab.2022.114738. Epub 2022 May 18.

Authors

Majid Arvand¹, Hoda Ilkhani², Mohamad Reza Ganjali³, Akram Pourhabib⁴

Affiliations

¹ Electroanalytical Chemistry Laboratory, Faculty of Chemistry, University of Guilan, Namjoo Street, P.O. Box: 1914-41335, Rasht, Iran. Electronic address: arvand@guilan.ac.ir.
² Central New Mexico Community College, Albuquerque, NM, 87106, USA.
³ Center of Excellence in Electrochemistry, Faculty of Chemistry, University of Tehran, Tehran, Iran.
⁴ Education Organization of Guilan Province, Farhang Square, P.O. Box: 41849-83111, Rasht, Iran.

PMID: 35597268
DOI: 10.1016/j.ab.2022.114738

Abstract

Metal ion-DNA interactions are important in nature as they often change the genetic material's structure and function. In this work, new Tb complex of TbCl₃ (tris(8-hydroxyquinoline-5-sulfonic acid) terbium) (Tb(QS)₃) was used as an electrochemical indicator for investigation of DNA-metal interaction and then a new DNA biosensor was designed using this complex. Cyclic voltammetry (CV) and differential pulse voltammetry (DPV) were used to study the interaction of Tb(QS)₃ with double-stranded DNA (ds-DNA). It was found that Tb(QS)₃ presented an excellent electrochemical activity on carbon paste electrode (CPE) and could intercalate into the double helix of double-stranded DNA. The interaction mechanism was elucidated in DNA solution and DNA modified carbon paste electrode by using differential pulse voltammetry and cyclic voltammetry. The binding ratio between this complex and ds-DNA was calculated to be 1:1. The extent of hybridization was evaluated on the basis of the difference between signals of Tb(QS)₃ with probe DNA before and after hybridization with complementary DNA. With this approach, target DNA could be detected in the range from 0.03 to 0.185 μM with detection limit of 0.021 μM. The interaction mode between Tb(QS)₃ and DNA was found to be mainly intercalative interaction.

Keywords: Electrochemical DNA biosensor; Hybridization; Metal ion-DNA interaction.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biosensing Techniques*
Carbon / chemistry
Coordination Complexes*
DNA / chemistry
Electrochemical Techniques
Electrodes
Nucleic Acid Hybridization

Substances

Coordination Complexes
Carbon
DNA