Enhancing harvest of biodiesel-promising microalgae using Daphnia domesticated by amino acids

Shiyu Yin; Wenbiao Jin; Xu Zhou; Wei Han; Shuhong Gao; Chuan Chen; Wanqing Ding; Zhongqi He; Yidi Chen; Guangming Jiang

doi:10.1016/j.envres.2022.113465

Enhancing harvest of biodiesel-promising microalgae using Daphnia domesticated by amino acids

Environ Res. 2022 Sep;212(Pt D):113465. doi: 10.1016/j.envres.2022.113465. Epub 2022 May 17.

Authors

Shiyu Yin¹, Wenbiao Jin², Xu Zhou³, Wei Han¹, Shuhong Gao¹, Chuan Chen⁴, Wanqing Ding¹, Zhongqi He¹, Yidi Chen¹, Guangming Jiang⁵

Affiliations

¹ State Key Lab of Urban Water Resource and Environment, School of Civil and Environmental Engineering, Harbin Institute of Technology Shenzhen, Shenzhen, 518055, China; Shenzhen Engineering Laboratory of Microalgae Bioenergy, Harbin Institute of Technology (Shenzhen), 518055, Shenzhen, China.
² State Key Lab of Urban Water Resource and Environment, School of Civil and Environmental Engineering, Harbin Institute of Technology Shenzhen, Shenzhen, 518055, China; Shenzhen Engineering Laboratory of Microalgae Bioenergy, Harbin Institute of Technology (Shenzhen), 518055, Shenzhen, China. Electronic address: jinwb@hit.edu.cn.
³ State Key Lab of Urban Water Resource and Environment, School of Civil and Environmental Engineering, Harbin Institute of Technology Shenzhen, Shenzhen, 518055, China; Shenzhen Engineering Laboratory of Microalgae Bioenergy, Harbin Institute of Technology (Shenzhen), 518055, Shenzhen, China. Electronic address: zhouxu@hit.edu.cn.
⁴ State Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, 150090, Harbin, China.
⁵ School of Civil, Mining and Environmental Engineering, University of Wollongong, NSW, 2522, Wollongong, Australia.

PMID: 35594959
DOI: 10.1016/j.envres.2022.113465

Abstract

Mass production of microalgal biodiesel is hindered by microalgae harvesting efficiency and costs. In this study, Daphnia domesticated by amino acids were used to harvest microalgae via ingesting. The main factors (density of Daphnia, salinity, pH, light-environment, temperature and algal concentration) that were conducive to Daphnia feeding were optimized. Under the optimal condition, Microalgae-feeding Daphnia were domesticated by adding D-glutamic acid and L-cysteine as stimulating factors. After that, the ingestion rate of domesticated Daphnia increased by 24.93%. The presence of Daphnia as a predator can induce microalgae to mass into clusters. Combining Daphnia feeding and the inductive defense flocculation of microalgae, the harvesting rate of mixed algae (Chlorella pyrenoidosa and Scenedesmus obliquus) reached over 95% after 9 h. Overall, this work suggested that Daphnia feeding process is a green and economical approach for microalgae harvesting.

Keywords: Amino acids; Domesticated Daphnia; Inducible defense; Microalgal harvesting; Predation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acids / metabolism
Animals
Biofuels
Biomass
Chlorella*
Daphnia
Flocculation
Microalgae* / metabolism

Substances

Amino Acids
Biofuels