MAECI: A pipeline for generating consensus sequence with nanopore sequencing long-read assembly and error correction

PLoS One. 2022 May 20;17(5):e0267066. doi: 10.1371/journal.pone.0267066. eCollection 2022.

Abstract

Nanopore sequencing produces long reads and offers unique advantages over next-generation sequencing, especially for the assembly of draft bacterial genomes with improved completeness. However, assembly errors can occur due to data characteristics and assembly algorithms. To address these issues, we developed MAECI, a pipeline for generating consensus sequences from multiple assemblies of the same nanopore sequencing data and error correction. Systematic evaluation showed that MAECI is an efficient and effective pipeline to improve the accuracy and completeness of bacterial genome assemblies. The available codes and implementation are at https://github.com/langjidong/MAECI.

MeSH terms

  • Consensus Sequence
  • Genomics
  • High-Throughput Nucleotide Sequencing
  • Nanopore Sequencing*
  • Sequence Analysis, DNA
  • Software

Grants and funding

The author(s) received no specific funding for this work.