SIBLING PAIR ANALYSIS IN THE IDENTIFICATION PROCESS OF THE MADURESE POPULATION USING STR CODIS LOCI

Ahmad Yudianto; Arofi Kurniawan; Fery Setiawan; Egita Windrianatama Puspa; Racy Youngest

doi:10.21010/Ajid.v16i2.5

SIBLING PAIR ANALYSIS IN THE IDENTIFICATION PROCESS OF THE MADURESE POPULATION USING STR CODIS LOCI

Afr J Infect Dis. 2022 May 6;16(2):42-54. doi: 10.21010/Ajid.v16i2.5. eCollection 2022.

Authors

Ahmad Yudianto^{1

2

3}, Arofi Kurniawan^{4

2}, Fery Setiawan^{2

5}, Egita Windrianatama Puspa², Racy Youngest²

Affiliations

¹ Department of Forensic Medicine and Medicolegal, Faculty of Medicine, Universitas Airlangga, Indonesia.
² Forensics Study Program, Magister Program of Postgraduate School, Universitas Airlangga, Indonesia.
³ Human Genetics Study Group, Institute of Tropical Diseases, Universitas Airlangga, Indonesia.
⁴ Department of Forensic Odontology, Faculty of Dental Medicine, Universitas Airlangga, Indonesia.
⁵ Doctoral Program of Medical Science, Faculty of Medicine, Universitas Airlangga, Indonesia.

Abstract

Background: The most common problem in forensic personal identification, particularly in paternity tests, is the availability of information originating from a mother or a father that can be used as a comparison in the forensic DNA examination process. The use of sibling analysis in paternity tests is still not widely known.

Materials and methods: The respondents of this study were 25 families of the Madurese population with 6, 9, and 10 sibling pairs categorized as female-female, male-male, and male-female siblings. The kinship relationship was analyzed by using 13 STR CODIS loci technique (TPOX, D3S1358, FGA, D5S818, CSFIPO, D7S820, D8S1179, THOI, vWA, D13S317, D16S539, D18S51, D21S11), and Amelogenin (Amel) x: 106bp,y: 112bp).

Results: The results of DNA contents and purity examination of the extracted DNA sample showed that the average value of DNA contents was 675±5.35 ng/μL with a purity range of 1.05-1.86. The findings proved that the male-male siblings of the Madurese population had the highest allele sharing percentage at the loci of D13S317, D16S539, and D21S11. The highest allele sharing percentage for female-female siblings was at the loci of TPOX and D21S11. Meanwhile, the male-female siblings had the highest allele sharing percentage at the loci of TPOX, D5S818, vWA, D7S820, THO1, vWA, and D13S317.

Conclusion: The main STR loci recommended in the male-female siblings of Madurese population identification are TPOX, D13S317, and D21S11.

Keywords: Human &; Identification; Madurese Population; Mortality; STR CODIS; Siblings.