Primary Macrophage Culture from Porcine Blood and Lungs

Lynnette C Goatley; Rachel Nash; Christopher L Netherton

doi:10.1007/978-1-0716-2333-6_4

Primary Macrophage Culture from Porcine Blood and Lungs

Methods Mol Biol. 2022:2503:63-72. doi: 10.1007/978-1-0716-2333-6_4.

Authors

Lynnette C Goatley¹, Rachel Nash², Christopher L Netherton³

Affiliations

¹ African Swine Fever Vaccinology Group, The Pirbright Institute, Pirbright, Woking, UK. lynnette.goatley@pirbright.ac.uk.
² Dig Worldwide, Sandwich, UK.
³ African Swine Fever Vaccinology Group, The Pirbright Institute, Pirbright, Woking, UK.

PMID: 35575886
DOI: 10.1007/978-1-0716-2333-6_4

Abstract

Primary cultures represent the most reliable method to isolate and propagate field isolates of African swine fever virus (ASFV ). Within the pig ASFV predominantly targets the reticuloendothelial system for replication; therefore, primary macrophage cell cultures are commonly used to isolate, propagate, and study the virus life cycle in the laboratory. In this chapter we will describe methods for the direct isolation of pulmonary alveolar macrophages by lung lavage and the culture of monocyte-derived macrophages from pig blood. We also include a method for the positive selection of CD14+ monocytes as a source for monocyte-derived macrophages from pig blood using microbeads.

Keywords: African swine fever; Buffy coat; Macrophage; Monocyte; Peripheral blood mononuclear cells; Virus.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

African Swine Fever Virus*
African Swine Fever*
Animals
Cells, Cultured
Lung
Macrophages
Swine

Abstract

Publication types

MeSH terms

Grants and funding