Complete chloroplast genome characterization and phylogenetic analysis of Clematis mandshurica (Ranunculaceae)

Yi Cui; Lihua Yang; Yanzhe Ding; Yingxin Sun; Jiao Wang; Yunfei Xi; Mei Han; Limin Yang; Zhongming Han; Yunhe Wang

doi:10.1080/23802359.2022.2073839

Complete chloroplast genome characterization and phylogenetic analysis of Clematis mandshurica (Ranunculaceae)

Mitochondrial DNA B Resour. 2022 May 10;7(5):822-824. doi: 10.1080/23802359.2022.2073839. eCollection 2022.

Authors

Yi Cui^{1

2}, Lihua Yang³, Yanzhe Ding^{1

2}, Yingxin Sun^{1

2}, Jiao Wang^{1

2}, Yunfei Xi^{1

2}, Mei Han^{1

2}, Limin Yang^{1

2}, Zhongming Han^{1

2}, Yunhe Wang^{1

2}

Affiliations

¹ College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun, China.
² State Key Laboratory of JLP-MOST for Ecological Restoration and Ecosystem Management, Changchun, China.
³ College of Life Science, Changchun Sci-Tech University, Changchun, China.

Abstract

The complete chloroplast genome sequence of Clematis mandshurica Ruprecht (1867), a specie of the Ranunculaceae family, and its phylogenetic relationships with other species have been reported in this study. The complete chloroplast genome of C. mandshurica is 159,563 bp in length, including a large single-copy (LSC) region of 79,360 bp, a small single-copy (SSC) region of 18,121 bp, and a pair of identical inverted repeat regions (IRs) of 31,041 bp. The genome encodes a total of 132 genes, including 90 protein-coding genes, 34 transfer RNA (tRNA) genes, and eight ribosomal RNA (rRNA) genes. The phylogenetic analysis reveals that C. mandshurica was found to be closest to Clematis taeguensis. The complete chloroplast genome of C. mandshurica contributes to a better understanding of phylogenetic relationships among Clematis species.

Keywords: Clematis mandshurica; chloroplast genome; phylogenetic analysis.

Grants and funding

This project is funded by the National Key R&D Program of China [2019YFC1710700], Scientific and Technological Planning Project of Jilin Province [20200404010YY, 20200708033YY], and China Agriculture Research System of MOF and MARA [CARS-21].