Mycodegradation of diazinon pesticide utilizing fungal strains isolated from polluted soil

Ashraf Abdel-Fattah Mostafa; Mohamed Taha Yassin; Turki M Dawoud; Fatimah O Al-Otibi; Shaban Rm Sayed

doi:10.1016/j.envres.2022.113421

Mycodegradation of diazinon pesticide utilizing fungal strains isolated from polluted soil

Environ Res. 2022 Sep;212(Pt C):113421. doi: 10.1016/j.envres.2022.113421. Epub 2022 May 12.

Authors

Ashraf Abdel-Fattah Mostafa¹, Mohamed Taha Yassin², Turki M Dawoud¹, Fatimah O Al-Otibi¹, Shaban Rm Sayed³

Affiliations

¹ Botany and Microbiology Department, College of Science, King Saud University, P.O. 2455, Riyadh, 11451, Saudi Arabia.
² Botany and Microbiology Department, College of Science, King Saud University, P.O. 2455, Riyadh, 11451, Saudi Arabia. Electronic address: myassin2.c@ksu.edu.sa.
³ Electron Microscope Unit, Collage of Science, King Saud University, Riyadh, 11451, Saudi Arabia.

PMID: 35568233
DOI: 10.1016/j.envres.2022.113421

Abstract

The current study aimed to isolate biodegradable soil fungi capable of metabolizing diazinon. The collected soil samples were investigated for diazinon pollution to detect the pesticide level in the polluted soil samples. Food poisoning techniques were utilized to preliminary investigate the biodegradation efficiency of the isolated fungal strains to diazinon pesticide using solid and liquid medium and also to detect their tolerance to different concentrations. GC-MS analysis of control and treated flasks were achieved to determine the diazinon residues for confirmation of the biodegradation efficiency. The total diazinon residues in the collected soil samples was found to be 0.106 mg/kg. Out of thirteen fungal strains isolated form diazinon polluted soils, six strains were potentially active in diazinon biodegradation. Food poisoning technique showed that A. niger, B. antennata, F. graminearum, P. digitatum, R. stolonifer and T. viride strains recorded fungal growth diameters of 65.2 ± 0.18, 57.5 ± 0.41, 47.2 ± 0.36, 56.5 ± 0.27, 85.0 ± 0.01, 85.0 ± 0.06 mm respectively in the treated group which were non significantly different compared to that of control (P > 0.05), indicating the high efficiency of these strains in diazinon degradation compared to the other isolated strains. GC-MS analysis revealed that B. antennata was the most efficient strain in diazinon degradation recording 32.24 ± 0.15 ppm concentration after 10 days incubation. Linear regression analysis confirmed that B. antennata was the most effective biodegradable strain recording the highest diazinon dissipation (83.88%) with the lowest T_1/2 value of 5.96 days while T. viride, A. niger, R. stolonifer and F. graminearum exhibited a high biodegradable activities reducing diazinon to 80.26%, 78.22%, 77.36% and 75.43% respectively after 10 days incubation. In conclusion, these tolerant fungi could be considered as promising, eco-friendly and biodegradable fungi for the efficient and potential removal of hazardous diazinon from polluted soil.

Keywords: Bioremediation; Diazinon; Fungal tolerance; GC-MS; Organophosphorus pesticides; Saprophytic fungi.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biodegradation, Environmental
Diazinon / analysis
Diazinon / chemistry
Diazinon / metabolism
Foodborne Diseases*
Fungi
Pesticides* / analysis
Soil / chemistry
Soil Microbiology
Soil Pollutants* / analysis

Substances

Pesticides
Soil
Soil Pollutants
Diazinon