The long-term stability of implants requires good peri-implant soft tissue sealing. Calcium ion (Ca2+ ) was loaded onto titanium surface by a hydrothermal method. In vitro, the morphology and composition of titanium surfaces were determined by scanning electron microscopy and energy-dispersive spectroscopy; proliferation of hGF-1 cells was measured by the CCK-8 assay; immunofluorescence staining was done to detect adherent proteins on titanium surface. In vivo, the degree of attachment between the implant and the surrounding soft tissue was measured by horseradish peroxidase (HRP). The percentage of hGF-1 cells adhering in the Ca group was significantly higher (p < .01); the fluorescence of integrin-β1 and F-actin in the Ca group was stronger; Ca group had the shorter length of HRP (p < .01). Ca2+ can be added to the surface of titanium by a hydrothermal method and it will be more beneficial for soft tissue early sealing. HIGHLIGHTS: A simple method to form micron crystals on titanium surface. The micron crystal promotes the attachment of human gingival fibroblasts. Facilitates early and rapid formation of soft-tissue sealing around implants.
Keywords: dental implants; human gingival fibroblasts; soft-tissue sealing.
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