Microfluidics-Based Time-Resolved Fluorescence Immunoassay for the On-Site Detection of Aflatoxins B1 Zearalenone and Deoxynivalenol in Cereals

Xu Wang; Disha Lu; Qingfeng Huang; Jinyi Yang

doi:10.3390/foods11091319

Microfluidics-Based Time-Resolved Fluorescence Immunoassay for the On-Site Detection of Aflatoxins B1 Zearalenone and Deoxynivalenol in Cereals

Foods. 2022 Apr 30;11(9):1319. doi: 10.3390/foods11091319.

Authors

Xu Wang¹, Disha Lu¹, Qingfeng Huang¹, Jinyi Yang¹

Affiliation

¹ Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, China.

Abstract

The primary pollutants in cereal products are aflatoxins B1 (AFB1), zearalenone (ZEN), and deoxynivalenol (DON). In this study, anti-AFB1 MAb (4C9), anti-ZEN MAb (2A3), and anti-DON MAb (1F10) were developed and used in time-resolved fluorescence immunoassay microfluidics to determine AFB1, ZEN, and DON in agricultural products. The linear range for AFB1, ZEN, and DON were 0.05~2.2 μg/kg, 1.45~375.75 μg/kg, and 11.1~124.2 μg/kg, respectively. In maize, the recoveries of AFB1/ZEN/DON were 92~101%, 102~105%, and 103~108%, respectively. High-performance liquid chromatography and the proposed approach had a good correlation. Time-resolved fluorescence immunoassay microfluidics is a highly efficient and sensitive field detection method for fungal toxins in agricultural products.

Keywords: aflatoxins B1; cereals; deoxynivalenol; microfluidics; monoclonal antibody; time-resolved fluorescence immunoassay; zearalenone.

Grants and funding

This work was supported financially by the National Key Research and Development Program of China (2018YFC1602904); Guangdong Province Basic and Applied Basic Research Project (2019B1515210025); Guangdong Province Basic and Applied Basic Research Project (2020A1515011238); Guangdong Province Basic and Applied Basic Research Project (2018B030314005).