The Phenotype of the Adipocytes Derived from Subcutaneous and Visceral ADMSCs Is Altered When They Originate from Morbidly Obese Women: Is There a Memory Effect?

Agnieszka Mikłosz; Bartłomiej Łukaszuk; Elżbieta Supruniuk; Kamil Grubczak; Aleksandra Starosz; Magdalena Kusaczuk; Monika Naumowicz; Adrian Chabowski

doi:10.3390/cells11091435

The Phenotype of the Adipocytes Derived from Subcutaneous and Visceral ADMSCs Is Altered When They Originate from Morbidly Obese Women: Is There a Memory Effect?

Cells. 2022 Apr 23;11(9):1435. doi: 10.3390/cells11091435.

Authors

Agnieszka Mikłosz¹, Bartłomiej Łukaszuk¹, Elżbieta Supruniuk¹, Kamil Grubczak², Aleksandra Starosz², Magdalena Kusaczuk³, Monika Naumowicz⁴, Adrian Chabowski¹

Affiliations

¹ Department of Physiology, Medical University of Bialystok, Mickiewicza 2C Street, 15-222 Bialystok, Poland.
² Department of Regenerative Medicine and Immune Regulation, Medical University of Bialystok, Waszyngtona 13 Street, 15-269 Bialystok, Poland.
³ Department of Pharmaceutical Biochemistry, Medical University of Bialystok, Mickiewicza 2A Street, 15-222 Bialystok, Poland.
⁴ Department of Physical Chemistry, Faculty of Chemistry, University of Bialystok, K. Ciolkowskiego 1K Street, 15-245 Bialystok, Poland.

Abstract

Adipose tissue is an abundant source of mesenchymal stem cells (ADMSCs). Evidence has suggested that depot-specific ADMSCs (obtained from subcutaneous or visceral adipose tissue-subADMSCs or visADMSCs, respectively) account for differential responses of each depot to metabolic challenges. However, little is known about the phenotype and changes in metabolism of the adipocytes derived from ADMSCs of obese individuals. Therefore, we investigated the phenotypic and metabolic characteristics, particularly the lipid profile, of fully differentiated adipocytes derived from ADMSCs of lean and obese (with/without metabolic syndrome) postmenopausal women. We observed a depot-specific pattern, with more pronounced changes present in the adipocytes obtained from subADMSCs. Namely, chronic oversupply of fatty acids (present in morbid obesity) triggered an increase in CD36/SR-B2 and FATP4 protein content (total and cell surface), which translated to an increased LCFA influx (³H-palmitate uptake). This was associated with the accumulation of TAG and DAG in these cells. Furthermore, we observed that the adipocytes of visADMSCs origin were larger and showed smaller granularity than their counterparts of subADMSCs descent. Although ADMSCs were cultured in vitro, in a fatty acids-deprived environment, obesity significantly influenced the functionality of the progenitor adipocytes, suggesting the existence of a memory effect.

Keywords: ADMSCs; adipocytes; lipid metabolism; metabolic syndrome; obesity; subcutaneous and visceral adipose tissue.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adipocytes / metabolism
Fatty Acids / metabolism
Female
Humans
Mesenchymal Stem Cells* / metabolism
Obesity, Morbid* / metabolism
Phenotype
Subcutaneous Fat

Substances

Fatty Acids