Due to the brittleness of gelatin, the resulting absence of mechanical performance restricts its applications in vascular tissue engineering. In this research, the fabrication of poly(ester-urethane) urea/gelatin (PU75) nanofibers via an electrospinning technique, followed by different crosslinking methods, resulted in the improvement of its mechanical properties. Poly(ester urethane) urea (PEUU) nanofibrous scaffolds and PU75-based nanofibrous scaffolds were characterized using scanning electron microscopy (SEM), attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy, wide-angle X-ray diffraction (WAXRD), a mechanical properties test, a cytocompatibility assay, a hemolysis assay, and a histological analysis. Water contact angle (WCA) tests confirmed that the PU75-GA (PU75 nanofibers crosslinked with glutaraldehyde vapor) nanofibrous scaffold surfaces became more hydrophilic compared with other crosslinked nanofibrous scaffolds. The results show that the PU75-GA nanofibrous scaffold exhibited a combination of excellent mechanical properties, suitable pore diameters, hydrophilic properties, good cytocompatibility, and reliable hemocompatibility. Overall, PU75-GA nanofibers may be a potential scaffold for artificial blood vessel construction.
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