Leonurus japonicus Houtt. is a herbaceous annual of the Lamiaceae family with pantropical distribution; it is called motherwort in China. It plays important roles in anticoagulation and antioxidation. This study aimed to explore the optimization of ultrasound-assisted extraction of multiple compounds from motherwort as well as their antioxidant and anticoagulation activities using response surface methodology. Box-Behnken design was employed to optimize three significant influences, namely extraction time, extraction temperature and ethanol concentration. The optimum extraction parameters acquired based on a combination of the yield of the target compounds and their antioxidant and anticoagulation activities were an extraction time of 38.2 min, an extraction temperature of 30.0 °C, an ethanol concentration of 48.9% (v/v), a solid-liquid ratio of 30.0 mL g-1 and an ultrasonic power of 500.0 W. Under the optimal conditions, the maximal yield of the anticoagulation and antioxidant compounds of motherwort was 0.994%; the thrombin time was 19.872 s; prothrombin time was 8.270 s; the activated partial thromboplastin time was 15.535 s; the fibrinogen was 1.420 g L-1; and the 1,1-diphenyl-2-picrylhydrazyl free radical scavenging activity was 1.503 mg mL-1. The optimized conditions model showed a good correlation between the predicted and experimental values. According to the results of our study, the optimization extraction significantly enabled study of the anticoagulation and antioxidant activities of compounds in motherwort; this may contribute to future research on the pharmacological activities of motherwort.
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