Acute Toxicity Bioassay and Determination of LC50 of Cadmium Chloride in Trichogaster (Colisa) fasciata

Appl Biochem Biotechnol. 2022 Sep;194(9):3890-3900. doi: 10.1007/s12010-022-03953-1. Epub 2022 May 12.

Abstract

Cadmium chloride is a metal salt used in industries in a large scale. Trichogaster (Colisa) fasciata (common name banded gourami) is an air-breathing fresh water fish with both nutritional and ornamental values. In this present study, as per EPA guidelines, acute toxicity bioassay was performed in a 96-h static test method in four replicates, each replicate having one control and five concentrations (25, 50, 75, 100 and 125 mg/L, respectively) of the metal salt. A total of 192 fish was used in the study in four series with each replicate containing 8 fish per concentration. The water temperature was maintained at 20-22℃ during the study. The data observed were analysed statistically on the basis of Finney's Probit Analysis method using SPSS software. The estimated 96-h LC50 value of cadmium chloride for the fish was 49.5 mg/L.

Keywords: Acute toxicity; Cadmium chloride; LC50; Static bioassay; Trichogaster fasciata.

MeSH terms

  • Animals
  • Biological Assay
  • Cadmium / toxicity
  • Cadmium Chloride / toxicity
  • Fishes
  • Fresh Water
  • Lethal Dose 50
  • Perciformes*
  • Toxicity Tests, Acute
  • Water Pollutants, Chemical*

Substances

  • Water Pollutants, Chemical
  • Cadmium
  • Cadmium Chloride