Genomic and Transcriptomic Analyses Reveal Pathways and Genes Associated With Brittle Stalk Phenotype in Maize

Jun Liu; Chuanbo Sun; Siqi Guo; Xiaohong Yin; Yuling Yuan; Bing Fan; Qingxue Lv; Xinru Cai; Yi Zhong; Yuanfeng Xia; Xiaomei Dong; Zhifu Guo; Guangshu Song; Wei Huang

doi:10.3389/fpls.2022.849421

Genomic and Transcriptomic Analyses Reveal Pathways and Genes Associated With Brittle Stalk Phenotype in Maize

Front Plant Sci. 2022 Apr 25:13:849421. doi: 10.3389/fpls.2022.849421. eCollection 2022.

Authors

Jun Liu¹, Chuanbo Sun¹, Siqi Guo², Xiaohong Yin¹, Yuling Yuan³, Bing Fan³, Qingxue Lv¹, Xinru Cai¹, Yi Zhong¹, Yuanfeng Xia¹, Xiaomei Dong², Zhifu Guo², Guangshu Song¹, Wei Huang¹

Affiliations

¹ Maize Research Institute, Jilin Academy of Agricultural Sciences, Gongzhuling, China.
² College of Biosciences and Biotechnology, Shenyang Agricultural University, Shenyang, China.
³ Hulun Buir Agricultural Reclamation Technology Development Co., Ltd., Hailar, China.

Abstract

The mechanical strength of the stalk affects the lodging resistance and digestibility of the stalk in maize. The molecular mechanisms regulating the brittleness of stalks in maize remain undefined. In this study, we constructed the maize brittle stalk mutant (bk5) by crossing the W22:Mu line with the Zheng 58 line. The brittle phenotype of the mutant bk5 existed in all of the plant organs after the five-leaf stage. Compared to wild-type (WT) plants, the sclerenchyma cells of bk5 stalks had a looser cell arrangement and thinner cell wall. Determination of cell wall composition showed that obvious differences in cellulose content, lignin content, starch content, and total soluble sugar were found between bk5 and WT stalks. Furthermore, we identified 226 differentially expressed genes (DEGs), with 164 genes significantly upregulated and 62 genes significantly downregulated in RNA-seq analysis. Some pathways related to cellulose and lignin synthesis, such as endocytosis and glycosylphosphatidylinositol (GPI)-anchored biosynthesis, were identified by the Kyoto Encyclopedia of Gene and Genomes (KEGG) and gene ontology (GO) analysis. In bulked-segregant sequence analysis (BSA-seq), we detected 2,931,692 high-quality Single Nucleotide Polymorphisms (SNPs) and identified five overlapped regions (11.2 Mb) containing 17 candidate genes with missense mutations or premature termination codons using the SNP-index methods. Some genes were involved in the cellulose synthesis-related genes such as ENTH/ANTH/VHS superfamily protein gene (endocytosis-related gene) and the lignin synthesis-related genes such as the cytochrome p450 gene. Some of these candidate genes identified from BSA-seq also existed with differential expression in RNA-seq analysis. These findings increase our understanding of the molecular mechanisms regulating the brittle stalk phenotype in maize.

Keywords: BSA-seq; RNA-seq; brittle stalk; cellulose and lignin synthesis; maize.