Duckweed systems for eutrophic water purification through converting wastewater nutrients to high-starch biomass: comparative evaluation of three different genera (Spirodela polyrhiza, Lemna minor and Landoltia punctata) in monoculture or polyculture

Guoke Chen; Yang Fang; Jun Huang; Yonggui Zhao; Qi Li; Fan Lai; Yaliang Xu; Xueping Tian; Kaize He; Yanling Jin; Li Tan; Hai Zhao

doi:10.1039/c8ra01856a

Duckweed systems for eutrophic water purification through converting wastewater nutrients to high-starch biomass: comparative evaluation of three different genera (Spirodela polyrhiza, Lemna minor and Landoltia punctata) in monoculture or polyculture

RSC Adv. 2018 May 16;8(32):17927-17937. doi: 10.1039/c8ra01856a. eCollection 2018 May 14.

Authors

Guoke Chen^{1

2

3}, Yang Fang^{1

3}, Jun Huang^{1

3}, Yonggui Zhao⁴, Qi Li^{1

2

3}, Fan Lai^{1

2

3}, Yaliang Xu^{1

2

3}, Xueping Tian^{1

2

3}, Kaize He^{1

3}, Yanling Jin^{1

3}, Li Tan^{1

3}, Hai Zhao^{1

3}

Affiliations

¹ Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, Chinese Academy of Sciences Chengdu 610041 China.
² University of Chinese Academy of Sciences Beijing 100049 China.
³ Environmental Microbiology Key Laboratory of Sichuan Province Chengdu 610041 China zhaohai@cib.ac.cn +86 28 82890733 +86 28 82890725.
⁴ Institute of Environmental Sciences and Ecological Restoration, School of Ecology and Environmental Sciences, Yunnan University Kunming 650091 China.

Abstract

The polyculture of different duckweed species is likely to integrate their advantages in removing pollutants and starch accumulation. Here, pilot-scale comparisons of three duckweed species (Spirodela polyrhiza K1, Lemna minor K2 and Landoltia punctata K3) in monoculture and polyculture were investigated. Results showed that the TN (total nitrogen) and TP (total phosphorus) in wastewater decreased from 6.0 and 0.56 mg L^-1 to below 0.5 and 0.1 mg L^-1, respectively. Namely, the water quality improved to Grade II under the Chinese standard. The highest TN and TP removal efficiencies were found to be 99.1% and 90.8% in the polyculture. Besides, the starch content of S. polyrhiza K1, L. minor K2, L. punctata K3 and the polyculture reached 24.8%, 32.3%, 39.3% and 36.3%, respectively. Accordingly, their average starch accumulation rates were 1.65, 2.15, 3.11 and 2.72 g m^-2 d^-1, respectively. Our results suggested that L. punctata K3 was a promising energy feedstock due to it having the highest starch production. The advantages of different duckweed species were investigated. In the polyculture, the pollutants were efficiently removed from wastewater, with a high starch accumulation. This study supplies a new insight into the application of duckweed in eutrophic water advanced treatment coupled with starch production.