A novel fluorescent probe for imaging the process of HOCl oxidation and Cys/Hcy reduction in living cells

Zhen Liu; Guoping Li; Yana Wang; Jiulong Li; Yang Mi; Linna Guo; Wenjian Xu; Dapeng Zou; Tiesheng Li; Yangjie Wu

doi:10.1039/c7ra13419c

A novel fluorescent probe for imaging the process of HOCl oxidation and Cys/Hcy reduction in living cells

RSC Adv. 2018 Mar 5;8(17):9519-9523. doi: 10.1039/c7ra13419c. eCollection 2018 Feb 28.

Authors

Zhen Liu¹, Guoping Li¹, Yana Wang¹, Jiulong Li¹, Yang Mi², Linna Guo¹, Wenjian Xu¹, Dapeng Zou¹, Tiesheng Li¹, Yangjie Wu¹

Affiliations

¹ The College of Chemistry and Molecular Engineering, Zhengzhou University Zhengzhou 450001 Henan Province P. R. China liguoping@zzu.edu.cn.
² School of Basic Medical Sciences, Zhengzhou University Zhengzhou 450001 Henan Province P. R. China.

Abstract

A new on-off-on fluorescent probe, CMOS, based on coumarin was developed to detect the process of hypochlorous acid (HOCl) oxidative stress and cysteine/homocysteine (Cys/Hcy) reduction. The probe exhibited a fast response, good sensitivity and selectivity. Moreover, it was applied for monitoring the redox process in living cells.