Gut modulatory effects of flaxseed derived Maillard reaction products in Sprague-Dawley rats during sub-chronic toxicity

An-Ran Zheng; Chao-Kun Wei; Zhi-Jing Ni; Kiran Thakur; Jian-Guo Zhang; Zhao-Jun Wei

doi:10.1016/j.fct.2022.113115

Gut modulatory effects of flaxseed derived Maillard reaction products in Sprague-Dawley rats during sub-chronic toxicity

Food Chem Toxicol. 2022 Jul:165:113115. doi: 10.1016/j.fct.2022.113115. Epub 2022 May 7.

Authors

An-Ran Zheng¹, Chao-Kun Wei², Zhi-Jing Ni³, Kiran Thakur⁴, Jian-Guo Zhang⁵, Zhao-Jun Wei⁶

Affiliations

¹ School of Food and Wine, Ningxia University, Yinchuan, 750021, People's Republic of China; School of Biological Science and Engineering, Ningxia Key Laboratory for the Development and Application of Microbial Resources in Extreme Environments, North Minzu University, Yinchuan, 750021, People's Republic of China. Electronic address: zhenganran17@163.com.
² School of Food and Wine, Ningxia University, Yinchuan, 750021, People's Republic of China; School of Biological Science and Engineering, Ningxia Key Laboratory for the Development and Application of Microbial Resources in Extreme Environments, North Minzu University, Yinchuan, 750021, People's Republic of China; School of Food and Biological Engineering, Hefei University of Technology, Hefei, 230009, People's Republic of China. Electronic address: weichaokun@nxu.edu.cn.
³ School of Biological Science and Engineering, Ningxia Key Laboratory for the Development and Application of Microbial Resources in Extreme Environments, North Minzu University, Yinchuan, 750021, People's Republic of China; School of Food and Biological Engineering, Hefei University of Technology, Hefei, 230009, People's Republic of China. Electronic address: lovebear@vip.163.com.
⁴ School of Biological Science and Engineering, Ningxia Key Laboratory for the Development and Application of Microbial Resources in Extreme Environments, North Minzu University, Yinchuan, 750021, People's Republic of China; School of Food and Biological Engineering, Hefei University of Technology, Hefei, 230009, People's Republic of China. Electronic address: kumarikiran@hfut.edu.cn.
⁵ School of Biological Science and Engineering, Ningxia Key Laboratory for the Development and Application of Microbial Resources in Extreme Environments, North Minzu University, Yinchuan, 750021, People's Republic of China; School of Food and Biological Engineering, Hefei University of Technology, Hefei, 230009, People's Republic of China. Electronic address: zhangjianguo@hfut.edu.cn.
⁶ School of Biological Science and Engineering, Ningxia Key Laboratory for the Development and Application of Microbial Resources in Extreme Environments, North Minzu University, Yinchuan, 750021, People's Republic of China; School of Food and Biological Engineering, Hefei University of Technology, Hefei, 230009, People's Republic of China. Electronic address: zjwei@hfut.edu.cn.

PMID: 35537647
DOI: 10.1016/j.fct.2022.113115

Abstract

Our study aimed to understand the effects of Maillard reaction products (MRPs) intake on intestinal health, in vitro digestion, and fermentation metabolites in Sprague-Dawley (SD) rats. MRPs promoted the digestion of pepsin, but was not conducive to the subsequent in vitro digestion of trypsin. MRPs ingestion increased the propionate in intestine, but it could not change the branched-chain fatty acids (BCFAs) and short-chain fatty acids (SCFAs). However, MRPs ingestion led to an increase in the Lactobacillus abundance in gut. In the high-dose groups, the abundance of genes in partial amino acid and monosaccharide metabolism increased, while in lipid metabolism decreased compared with the middle dose groups. Therefore, the absorption of MRPs was lowered than that of protein and carbohydrates. Through functional predictive analysis, our study could reveal the effects of long-term intake of MRPs on intestinal health in SD rats.

Keywords: Fermentation metabolites; Flaxseed; Functional prediction; Intestinal bacteria; Maillard reaction products; Sub-chronic toxicity.

MeSH terms

Amino Acids / chemistry
Animals
Flax*
Glycation End Products, Advanced* / metabolism
Maillard Reaction
Rats
Rats, Sprague-Dawley

Substances

Amino Acids
Glycation End Products, Advanced