Effects of pile driving sound playbacks and cadmium co-exposure on the early life stage development of the Norway lobster, Nephrops norvegicus

C A Stenton; E L Bolger; M Michenot; J A Dodd; M A Wale; R A Briers; M G J Hartl; K Diele

doi:10.1016/j.marpolbul.2022.113667

Effects of pile driving sound playbacks and cadmium co-exposure on the early life stage development of the Norway lobster, Nephrops norvegicus

Mar Pollut Bull. 2022 Jun:179:113667. doi: 10.1016/j.marpolbul.2022.113667. Epub 2022 May 6.

Authors

C A Stenton¹, E L Bolger², M Michenot³, J A Dodd⁴, M A Wale², R A Briers⁴, M G J Hartl⁵, K Diele⁶

Affiliations

¹ Aquatic Noise Research Group, School of Applied Sciences, Edinburgh Napier University, 9 Sighthill Court, Edinburgh EH11 4BN, UK; Centre for Conservation and Restoration Science, Edinburgh Napier University, 9 Sighthill Court, Edinburgh EH11 4BN, UK; St Abbs Marine Station, The Harbour, St Abbs, Eyemouth TD14 5PW, UK; Ocean Science Consulting Ltd., Spott Road, Dunbar EH42 1RR, UK. Electronic address: c.stenton@napier.ac.uk.
² Aquatic Noise Research Group, School of Applied Sciences, Edinburgh Napier University, 9 Sighthill Court, Edinburgh EH11 4BN, UK; Centre for Conservation and Restoration Science, Edinburgh Napier University, 9 Sighthill Court, Edinburgh EH11 4BN, UK; St Abbs Marine Station, The Harbour, St Abbs, Eyemouth TD14 5PW, UK.
³ École Nationale des Travaux Publics de L'état, 3 Rue Maurice Audin, 69 120 Vaulx en Velin, France.
⁴ Aquatic Noise Research Group, School of Applied Sciences, Edinburgh Napier University, 9 Sighthill Court, Edinburgh EH11 4BN, UK; Centre for Conservation and Restoration Science, Edinburgh Napier University, 9 Sighthill Court, Edinburgh EH11 4BN, UK.
⁵ Centre for Marine Biodiversity & Biotechnology, Institute of Life and Earth Sciences, School of Energy, Geoscience, Infrastructure & Society, Heriot-Watt University, Edinburgh EH14 4AS, UK.
⁶ Aquatic Noise Research Group, School of Applied Sciences, Edinburgh Napier University, 9 Sighthill Court, Edinburgh EH11 4BN, UK; Centre for Conservation and Restoration Science, Edinburgh Napier University, 9 Sighthill Court, Edinburgh EH11 4BN, UK; St Abbs Marine Station, The Harbour, St Abbs, Eyemouth TD14 5PW, UK. Electronic address: k.diele@napier.ac.uk.

PMID: 35533617
DOI: 10.1016/j.marpolbul.2022.113667

Abstract

There is an urgent need to understand how organisms respond to multiple, potentially interacting drivers in today's world. The effects of the pollutants anthropogenic sound (pile driving sound playbacks) and waterborne cadmium were investigated across multiple levels of biology in larval and juvenile Norway lobster, Nephrops norvegicus under controlled laboratory conditions. The combination of pile driving playbacks (170 dB_pk-pk re 1 μPa) and cadmium combined synergistically at concentrations >9.62 μg_[Cd] L^-1 resulting in increased larval mortality, with sound playbacks otherwise being antagonistic to cadmium toxicity. Exposure to 63.52 μg_[Cd] L^-1 caused significant delays in larval development, dropping to 6.48 μg_[Cd] L^-1 in the presence of piling playbacks. Pre-exposure to the combination of piling playbacks and 6.48 μg_[Cd] L^-1 led to significant differences in the swimming behaviour of the first juvenile stage. Biomarker analysis suggested oxidative stress as the mechanism resultant deleterious effects, with cellular metallothionein (MT) being the predominant protective mechanism.

Keywords: Biomarkers; Larvae; Metallothionein; Multiple drivers; Noise; Oxidative stress.

MeSH terms

Animals
Cadmium* / toxicity
Metallothionein
Nephropidae*
Noise
Sound
Sound Spectrography

Substances

Cadmium
Metallothionein