Screening of multi-class antibiotics in pork meat by LC-Orbitrap-MS with modified QuEChERS extraction

Qing Chen; Xiao-Dong Pan; Bai-Fen Huang; Jian-Long Han; Biao Zhou

doi:10.1039/c9ra04853g

Screening of multi-class antibiotics in pork meat by LC-Orbitrap-MS with modified QuEChERS extraction

RSC Adv. 2019 Sep 6;9(48):28119-28125. doi: 10.1039/c9ra04853g. eCollection 2019 Sep 3.

Authors

Qing Chen¹, Xiao-Dong Pan¹, Bai-Fen Huang¹, Jian-Long Han¹, Biao Zhou¹

Affiliation

¹ Zhejiang Provincial Center for Disease Control and Prevention, Institute of Physical-Chemistry and Toxicity Room No. 401, Bin-Sheng Road No. 3399, Binjiang District Hangzhou 310051 China zjupanxiaodong@hotmail.com +86 571 87115261 +86 571 87115274.

Abstract

The quantification capability of high resolution mass spectrometry is of great interest to analysts. We described a method for analysis of multi-class antibiotics in pork meat by UPLC-quadrupole (Q)-Orbitrap-MS. The QuEChERS approach with a clean-up step using a sorbent of primary-secondary amine (PSA) and C18 was adopted for sample preparation, and 37 antibiotics including beta-lactams, tetracyclines, sulfonamides, fluoroquinolones and macrolides were analyzed. The Q-Orbitrap method showed high sensitivity with limits of detection (LODs) ranging from 0.8 μg kg^-1 to 2.9 μg kg^-1. The method was further validated by intra and inter-day tests with fortified samples. Recovery (85-105.6%) and precision values (RSDs < 15%) for all analytes were obtained. The result indicates that UPLC-Q-Orbitrap-MS coupled with QuEChERS preparation can serve as a routine method for multi-class antibiotic analysis in pork meat.