Investigating Plant Biosynthetic Pathways Using Heterologous Gene Expression: Yeast as a Heterologous Host

Shanhui Xu; Sheng Wu; Yanran Li

doi:10.1007/978-1-0716-2273-5_19

Investigating Plant Biosynthetic Pathways Using Heterologous Gene Expression: Yeast as a Heterologous Host

Methods Mol Biol. 2022:2489:369-393. doi: 10.1007/978-1-0716-2273-5_19.

Authors

Shanhui Xu^#¹, Sheng Wu^#¹, Yanran Li²

Affiliations

¹ Department of Chemical and Environmental Engineering, University of California, Riverside, CA, USA.
² Department of Chemical and Environmental Engineering, University of California, Riverside, CA, USA. yanranl@ucr.edu.

^# Contributed equally.

PMID: 35524060
DOI: 10.1007/978-1-0716-2273-5_19

Abstract

Plant natural products (PNPs) are valuable resources for the development of pharmaceuticals and agrochemicals, yet the biosynthesis and metabolism of PNPs are largely unknown. Heterologous pathway reconstitution is a heavily adopted strategy in secondary metabolism characterization. Yeast systems have been broadly utilized in the heterologous production of PNPs and have been considered as a promising platform to investigate plant biosynthetic pathways. Here, we describe the reconstitution and verification of the upstream part of brassinolide biosynthesis in S. cerevisiae using this method.

Keywords: Biosynthesis; Heterologous gene expression; Metabolic engineering; Natural products; Saccharomyces cerevisiae; Yeast.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Biological Products* / metabolism
Biosynthetic Pathways* / genetics
Gene Expression
Metabolic Engineering / methods
Plants / genetics
Plants / metabolism
Saccharomyces cerevisiae / genetics
Saccharomyces cerevisiae / metabolism

Substances

Biological Products

Grants and funding

DP2 AT011445/AT/NCCIH NIH HHS/United States