Multi-omics analysis reveals the influence of tetracycline on the growth of ryegrass root

Tao Han; Zhaorong Mi; Zhe Chen; Jinjin Zhao; Haiguang Zhang; Yang Lv; Shouyang Du; Ruifang Bu; Junguo Zhou; Xinzheng Li; Zhiqiang Sun; Zhuo Chen; Yiting Song; Jun Zhang; Ruijuan Hao; Shidong Ge

doi:10.1016/j.jhazmat.2022.129019

Multi-omics analysis reveals the influence of tetracycline on the growth of ryegrass root

J Hazard Mater. 2022 Aug 5:435:129019. doi: 10.1016/j.jhazmat.2022.129019. Epub 2022 Apr 29.

Authors

Tao Han¹, Zhaorong Mi², Zhe Chen³, Jinjin Zhao⁴, Haiguang Zhang⁴, Yang Lv⁵, Shouyang Du⁶, Ruifang Bu², Junguo Zhou², Xinzheng Li², Zhiqiang Sun⁷, Zhuo Chen⁸, Yiting Song⁸, Jun Zhang⁸, Ruijuan Hao⁸, Shidong Ge⁹

Affiliations

¹ School of Horticulture and Landscape Architecture, Henan Institute of Science and Technology, Xinxiang 453003, China; Henan Province Engineering Research Center of Horticultural Plant Resource Utilization and Germplasm Enhancement, Henan Institute of Science and Technology, Xinxiang 453003, China; Postdoctoral Research Station, Henan Agricultural University, Zhengzhou 450002, China.
² School of Horticulture and Landscape Architecture, Henan Institute of Science and Technology, Xinxiang 453003, China; Henan Province Engineering Research Center of Horticultural Plant Resource Utilization and Germplasm Enhancement, Henan Institute of Science and Technology, Xinxiang 453003, China.
³ College of Life Science, Qinghai Normal University, Xining 810008, China.
⁴ The First Affiliated Hospital of Xinxiang Medical University, Weihui 453100, China.
⁵ Shanghai Fengyuan Biotechnology Limited Company, Shanghai 200240, China.
⁶ College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang 453003, China.
⁷ College of Horticulture, Henan Agricultural University, Zhengzhou 450002, China.
⁸ School of Horticulture and Landscape Architecture, Henan Institute of Science and Technology, Xinxiang 453003, China.
⁹ College of Landscape Architecture and Art, Henan Agricultural University, Zhengzhou 450002, China. Electronic address: shidongge@henau.edu.cn.

PMID: 35523093
DOI: 10.1016/j.jhazmat.2022.129019

Abstract

Environmental factors, including antibiotics such as tetracycline, can alter biological processes in plants. To ascertain how cell/tissue response to tetracycline, a multi-omic analysis was implemented to explore the molecular mechanism of tetracycline influencing the growth of ryegrass root. Tetracycline induced extensive changes in the root metabolome in plants, particularly impacting metabolites of flavonoid metabolic pathways, which were supported through consistent differences between transcriptome and proteome. Cross-comparison between mRNA and protein contents considered the authentication of congruence with related metabolites and revealed changes of several biological processes under tetracycline stress. Overall, we present an undemanding multi-omic strategy to survey the significant influence on the root under tetracycline stress.

Keywords: Metabolome; Proteome; Ryegrass root; Tetracycline; Transcriptome.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / toxicity
Biological Phenomena*
Lolium*
Metabolome
Tetracycline / toxicity
Transcriptome

Substances

Anti-Bacterial Agents
Tetracycline