Effect of dietary incorporation of peanut and linseed meals with or without enzyme mixture on physiological performance of broilers

Ahmed A Saleh; Amin Nahla; Khairy Amber; Nemeet Badawi; Salama M Aboelenin; Mohammed H Alzawqari; Sarah Albogami; Abdel-Moneim Eid Abdel-Moneim; Mohamed M Soliman; Mustafa Shukry

doi:10.1016/j.sjbs.2022.103291

Effect of dietary incorporation of peanut and linseed meals with or without enzyme mixture on physiological performance of broilers

Saudi J Biol Sci. 2022 Jun;29(6):103291. doi: 10.1016/j.sjbs.2022.103291. Epub 2022 Apr 21.

Authors

Affiliations

¹ Department of Poultry Production, Faculty of Agriculture, Kafrelsheikh University, 333516 Kafrelsheikh, Egypt.
² Biology Department, Turabah University College, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia.
³ Department of Animal Production, Faculty of Agriculture and Food Sciences, Ibb University, P.O. Box 70270, Ibb, Yemen.
⁴ Department of Biotechnology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia.
⁵ Biological Applications Department, Nuclear Research Center, Egyptian Atomic Energy Authority, 13759, Egypt.
⁶ Clinical Laboratory Sciences Department, Turabah University College, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia.
⁷ Department of Physiology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh 33516, Egypt.

Abstract

The present study aimed to evaluate the impact of feeding peanut meal and linseed meal (LSM) with or without enzyme mixture on growth, plasma metabolites, muscle amino acid (AA) profile, nutrient digestibility, and expression of nutrient absorption-related genes in broilers. A total of 560 one-day-old Cobb-500 male broiler chicks were distributed into eight experimental treatments (7 replications of 10 chicks each) as follows: This study was designed by using 560 one-day-old Cobb-500 male broiler chicks were distributed into eight experimental groups (7 replications of 10 chicks each) to evaluate the differences in body weight, body weight gain, feed intake, feed conversion rate, carcass parts, blood biochemical and mRNA expression genes. Group 1 (C) control fed the basal diet without supplements, Group 2 (C + E) is control group fed on 350 g/ton enzyme mixture, Group 3 (C + PNM100) is control group fed 100 kg/ton peanut meal, Group 4 (C + E + PNM100) is a control group fed on 350 g/ton enzyme mixture and 100 kg/ton peanut meal, Group 5 (C + LSM100) is a control group fed on 100 kg/ton linseed meal, Group 6 (C + E + LSM100) is a control group fed on 350 g/ton enzyme mixture and 100 kg/ton linseed meal, Group 7 (C + PNM50 + LSM50) is control group fed on 50 kg/ton peanut meal and 50 kg/ton linseed meal. Group 8 (C + E + PNM50 + LSM50) is the control group fed on 50 kg/ton peanut meal and 50 kg/ton linseed meal. Each gram of the enzyme mixture contains 11,000 U Xylanase, 6000 U Cellulase, 700 U β-Mannanase, 1500 U Phytase, 5 mg α-Amylase, and 2 mg Protease. No differences in Bodyweight, Bodyweight gain, Feed intake, and carcass parts were noticed among experimental groups, while abdominal fat (%) and FCR were reduced (P < 0.05) in PNM50 + LSM50 + E and LSM100 groups. Plasma metabolites were not altered except total cholesterol, triglyceride, and LDL, reduced (P < 0.01) in treated birds. Dietary inclusion of 100 kg PNM or LSM reduced (P < 0.05) methionine concentration in muscle, while all remaining AA and ammonia concentrations were unaffected. Hepatic MDA contents were reduced (P < 0.001) in treated groups. Nutrient digestibility was not altered among groups except for protein digestibility, which was elevated (P < 0.05) in PNM50 + LSM50 + E, E, and PNM100 + E groups. The highest mRNA expressions of PepT1, APN, SGLT1, HMGCR, GHr, and IGF-1 genes were noticed in PNM50 + LSM50 + E. Conclusively, PNM and LSM can efficiently substitute corn and soybean meal in broiler diets, particularly when fortified with exogenous enzymes, without negative impacts on broiler performance.

Keywords: Broilers; Gene expression; Growth performance; Linseed meal; Muscle AAs profile; Peanut meal.