Identification of Stable and Multiple Environment Interaction QTLs and Candidate Genes for Fiber Productive Traits Under Irrigated and Water Stress Conditions Using Intraspecific RILs of Gossypium hirsutum var. MCU5 X TCH1218

Narayanan Manikanda Boopathi; Gopal Ji Tiwari; Satya Narayan Jena; Kemparaj Nandhini; V K I Sri Subalakhshmi; Pilla Shyamala; Babita Joshi; Nallathambi Premalatha; S Rajeswari

doi:10.3389/fpls.2022.851504

Identification of Stable and Multiple Environment Interaction QTLs and Candidate Genes for Fiber Productive Traits Under Irrigated and Water Stress Conditions Using Intraspecific RILs of Gossypium hirsutum var. MCU5 X TCH1218

Front Plant Sci. 2022 Apr 18:13:851504. doi: 10.3389/fpls.2022.851504. eCollection 2022.

Authors

Narayanan Manikanda Boopathi¹, Gopal Ji Tiwari², Satya Narayan Jena², Kemparaj Nandhini³, V K I Sri Subalakhshmi³, Pilla Shyamala¹, Babita Joshi^{2

4}, Nallathambi Premalatha³, S Rajeswari³

Affiliations

¹ Department of Plant Biotechnology, CPMB&B, Tamil Nadu Agricultural University, Coimbatore, India.
² Plant Molecular Genetics Laboratory, CSIR-National Botanical Research Institute, Lucknow, India.
³ Department of Cotton, CPBG, Tamil Nadu Agricultural University, Coimbatore, India.
⁴ Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, India.

Abstract

Cotton productivity under water-stressed conditions is controlled by multiple quantitative trait loci (QTL). Enhancement of these productivity traits under water deficit stress is crucial for the genetic improvement of upland cotton, Gossypium hirsutum. In the present study, we constructed a genetic map with 504 single nucleotide polymorphisms (SNPs) covering a total span length of 4,416 cM with an average inter-marker distance of 8.76 cM. A total of 181 intra-specific recombinant inbred lines (RILs) were derived from a cross between G. hirsutum var. MCU5 and TCH1218 were used. Although 2,457 polymorphic SNPs were detected between the parents using the CottonSNP50K assay, only 504 SNPs were found to be useful for the construction of the genetic map. In the SNP genotyping, a large number of SNPs showed either >20% missing data, duplication, or segregation distortion. However, the mapped SNPs of this study showed collinearity with the physical map of the reference genome (G. hirsutum var.TM-1), indicating that there was no chromosomal rearrangement within the studied mapping population. RILs were evaluated under multi-environments and seasons for which the phenotypic data were acquired. A total of 53 QTL controlling plant height (PH), number of sympodial branches, boll number (BN), and boll weight (BW) were dissected by QTL analysis under irrigated and water stress conditions. Additionally, it was found that nine QTL hot spots not only co-localized for more than one investigated trait but were also stable with major QTL, i.e., with > 10% of phenotypic variation. One QTL hotspot on chromosome 22 flanked by AX-182254626-AX-182264770 with a span length of 89.4 cM co-localized with seven major and stable QTL linked to a number of sympodial branches both under irrigated and water stress conditions. In addition, putative candidate genes associated with water stress in the QTL hotspots were identified. Besides, few QTL from the hotspots were previously reported across various genetic architects in cotton validating the potential applications of these identified QTL for cotton breeding and improvement. Thus, the major and stable QTL identified in the present study would improve the cotton productivity under water-limited environments through marker-assisted selection.

Keywords: QTL; SNP; drought; genetic map; intra-specific cross; productive traits; upland cotton.