Aza-BODIPY probe for selective visualization of cyclooxygenase-2 in cancer cells

Thitima Pewklang; Kantapat Chansaenpak; Rung-Yi Lai; Parinya Noisa; Anyanee Kamkaew

doi:10.1039/c9ra01948k

Aza-BODIPY probe for selective visualization of cyclooxygenase-2 in cancer cells

RSC Adv. 2019 Apr 30;9(24):13372-13377. doi: 10.1039/c9ra01948k.

Authors

Thitima Pewklang¹, Kantapat Chansaenpak², Rung-Yi Lai¹, Parinya Noisa³, Anyanee Kamkaew¹

Affiliations

¹ School of Chemistry, Institute of Science, Suranaree University of Technology Nakhon Ratchasima Thailand 30000 anyanee@sut.ac.th.
² National Nanotechnology Center, National Science and Technology Development Agency, Thailand Science Park Pathum Thani Thailand 12120.
³ Laboratory of Cell-Based Assays and Innovations, School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology Nakhon Ratchasima Thailand 30000.

Abstract

AZB-IMC₂ was developed as a COX-2 specific probe that exhibited a brighter fluorescence signal in cancer cells that overexpress COX-2 compared to normal cells. Oxidative stress agent-treated inflamed cell lines inducing high COX-2 levels revealed an enhanced fluorescence signal. Inhibitory studies showed a markedly reduced fluorescence intensity in cancer cells. The results suggested that AZB-IMC₂ could be developed as a promising molecular tool for imaging guiding during surgery.