We describe a complete open-source hardware/software solution for high performance thermostatted peptide fraction collection to support mass spectrometry experiments with complex proteomes. The instrument is easy to assemble using parts readily available through retail channels at a fraction of the cost compared to typical commercial systems. Control software is written in Python allowing for rapid customization. We demonstrate several useful applications, including the automated deposition of LC separated peptides for matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) as well as collection and concatenation of peptide fractions from nanoflow HPLC separations.
Keywords: Fraction collection; HPLC, high pressure liquid chromatography; LC, liquid chromatography; LC-MALDI; LC-MS, Liquid Chromatography/Mass spectrometry; MALDI-MS, matrix-assisted laser desorption ionization mass spectrometry; MS, mass spectrometer/mass spectrometry; OEM, Original Equipment Manufacturer; Open source; Proteomics; Python.
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