Label-free quantitative proteomics to investigate the response of strawberry fruit after controlled ozone treatment

Cunkun Chen; Xiaojun Zhang; Huijie Zhang; Zhaojun Ban; Li Li; Chenghu Dong; Haipeng Ji; Wentong Xue

doi:10.1039/c8ra08405j

Label-free quantitative proteomics to investigate the response of strawberry fruit after controlled ozone treatment

RSC Adv. 2019 Jan 3;9(2):676-689. doi: 10.1039/c8ra08405j. eCollection 2019 Jan 2.

Authors

Cunkun Chen^{1

2}, Xiaojun Zhang³, Huijie Zhang⁴, Zhaojun Ban⁵, Li Li⁶, Chenghu Dong³, Haipeng Ji³, Wentong Xue^{1

2}

Affiliations

¹ Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University Beijing China 17806283010@163.com.
² College of Food Science and Nutritional Engineering, China Agricultural University Beijing China.
³ National Engineering Technology Research Center for Preservation of Agricultural Products, Key Laboratory of Postharvest Physiology and Storage of Agricultural Products, Ministry of Agriculture of China, Tianjin Key Laboratory of Postharvest Physiology and Storage of Agricultural Products Tianjin China.
⁴ College of Food Engineering and Biotechnology, Tianjin University of Science and Technology Tianjin China.
⁵ Zhejiang Provincial Key Laboratory of Chemical and Biological Processing Technology of Farm Products, Zhejiang University of Science and Technology Hangzhou China.
⁶ Key Laboratory for Agro-Products Postharvest Handling of Ministry of Agriculture, Zhejiang Key Laboratory for Agro-Food Processing, College of Biosystems Engineering and Food Science, Zhejiang University Hangzhou China.

Abstract

To elucidate postharvest senescence in strawberry (Fragaria ananassa Duch. var. 'JingTaoXiang') fruit in response to ozone treatment at different concentrations (0, 2.144, 6.432, and 10.72 mg m^-3), a label-free quantitative proteomic investigation was performed. Postharvest physiological quality traits including respiration rate, firmness, titratable acid, and anthocyanin content were characterized. The observed protein expression profile after storage was related to delayed senescence in strawberries. A total of 2413 proteins were identified in differentially treated strawberry fruits, and 382 proteins were differentially expressed between the four treatments on day 7 and the initial value (blank 0). Proteins related to carbohydrate and energy metabolism and anthocyanin biosynthesis, cell stress response, and fruit firmness were characterized and quantified. Ozone treatment at the concentration of 10.72 mg m^-3 effectively delayed the senescence of the strawberry. The proteomic profiles were linked to physiological traits of strawberry fruit senescence to provide new insights into possible molecular mechanisms.