Polyphenols extracted from huangjiu have anti-inflammatory activity in lipopolysaccharide stimulated RAW264.7 cells

Lin Peng; Aisikaer Ai-Lati; Zhongwei Ji; Shuguang Chen; Jian Mao

doi:10.1039/c8ra09671f

Polyphenols extracted from huangjiu have anti-inflammatory activity in lipopolysaccharide stimulated RAW264.7 cells

RSC Adv. 2019 Feb 12;9(10):5295-5301. doi: 10.1039/c8ra09671f. eCollection 2019 Feb 11.

Authors

Lin Peng^{1

2

3}, Aisikaer Ai-Lati^{1

2

3}, Zhongwei Ji^{1

2

3}, Shuguang Chen⁴, Jian Mao^{1

2

3

5}

Affiliations

¹ National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University Wuxi 214122 China maojian@jiangnan.edu.cn.
² School of Food Science and Technology, Jiangnan University Wuxi Jiangsu 214122 China.
³ National Engineering Research Center of Chinese Rice Wine Shaoxing Zhejiang 31200 China.
⁴ Department of General Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College (CAMS & PUMC) Beijing 100730 China cshuguang6@sina.com.
⁵ State Key Laboratory of Food Science & Technology, Jiangnan University Wuxi Jiangsu 214122 China.

Abstract

In the present study, an extraction method, combining extraction by ethyl acetate + ethanol and purification by HPD400 resin, was established to obtain huangjiu polyphenol extract (HPE). After extraction and purification, the polyphenol yield was 22.57%, and 90.57% protein and 97.99% sugar were removed. HPLC analysis indicated that (+)-catechin (91.33 μg mL^-1) was the predominant phenolic compound among the 11 detected polyphenols. In LPS-stimulated RAW264.7 cells, HPE exhibits anti-inflammatory effects by inhibiting the production of NO and pro-inflammatory cytokines (TNF-α, interleukin IL-6 and IL-1β). The anti-inflammatory effect of HPE is associated with the inhibition of iNOS expression, the suppression of NF-κB translocation to the nucleus, and the inhibition of the phosphorylation of IκB and the MAPK family proteins, e.g. p-38, Erk 1/2, and JNK. Moreover, the activation of Nrf2 and HO-1 is also related to the anti-inflammatory effect of HPE.