Enzyme immobilisation on poly-l-lysine-containing calcium phosphate particles for highly sensitive glucose detection

Suzuka Kojima; Fukue Nagata; Masahiko Inagaki; Shinichi Kugimiya; Katsuya Kato

doi:10.1039/c9ra01764j

Enzyme immobilisation on poly-l-lysine-containing calcium phosphate particles for highly sensitive glucose detection

RSC Adv. 2019 Apr 8;9(19):10832-10841. doi: 10.1039/c9ra01764j. eCollection 2019 Apr 3.

Authors

Suzuka Kojima^{1

2}, Fukue Nagata¹, Masahiko Inagaki^{1

2}, Shinichi Kugimiya², Katsuya Kato¹

Affiliations

¹ National Institute of Advanced Industrial Science and Technology 2266-98, Anagahora, Shimo-Shidami, Moriyama-ku Nagoya Aichi 463-8560 Japan katsuya-kato@aist.go.jp +81 52 736 7405 +81 52 736 7551.
² Materials Chemistry Course, Graduate School of Engineering, Aichi Institute of Technology 1247 Yachigusa, Yakusa-cho Toyota Aichi 470-0392 Japan suzuka-kojima@aist.go.jp.

Abstract

High catalytic activities of enzymes are necessary for enzyme immobilising technology for the development of glucose sensors. The aim of this study is to synthesise two types of poly(l-lysine)-containing calcium phosphate particles (pLys-HAp) and to achieve the immobilisation of glucose oxidase (GOX) on them. The oxidation activity of GOX immobilised on these particles was more than 80% compared to that of native GOX (considered to be 100%). Additionally, the relative activity of GOX immobilised on poly-ε-lysine-containing HAp (ε-pLys-HAp) remained approximately 70% after ten cycles. Moreover, glucose detection was able to be performed in the linear range of 4-400 μM using GOX immobilised on pLys-HAp composites. In the direct electrochemistry measurement using the cyclic voltammetry (CV) method, a glassy carbon electrode (GCE) modified by ε-pLys-HAp was a good enzyme electrode and can be used for glucose detection with high sensitivity. From these results, poly(l-lysine)-containing HAp composites can be expected to be enzyme immobilisation agents with high stability and biosensors with high sensitivity.