Data on complete genome sequence and annotation of two multidrug resistant atypical enteropathogenic Escherichia coli O177 serotype isolated from cattle faeces

Peter Kotsoana Montso; Victor Mlambo; Collins Njie Ateba

doi:10.1016/j.dib.2022.108167

Data on complete genome sequence and annotation of two multidrug resistant atypical enteropathogenic Escherichia coli O177 serotype isolated from cattle faeces

Data Brief. 2022 Apr 14:42:108167. doi: 10.1016/j.dib.2022.108167. eCollection 2022 Jun.

Authors

Peter Kotsoana Montso^{1

2}, Victor Mlambo³, Collins Njie Ateba^{1

2}

Affiliations

¹ Food Security and Safety Niche Area, Faculty of Natural and Agricultural Sciences, North-West University - Mafikeng Campus, Private Bag X2046, Mmabatho 2735, South Africa.
² Bacteriophage Therapy and Phage Bio-Control Laboratory, Department of Microbiology, Faculty of Natural and Agricultural Sciences, North-West University- Mafikeng Campus, Private Bag X2046, Mmabatho 2735, South Africa.
³ School of Agricultural Sciences, Faculty of Agriculture and Natural Sciences, University of Mpumalanga, Private Bag X11283, Mbombela 1200, South Africa.

Abstract

Atypical enteropathogenic E. coli belonging to the serotype O177 is a rare strain found in ruminants, especially cattle. When compared to shiga toxin producing E. coli (STEC) O157 and non-O157 STEC (O26, O45, O103, O104, O111, O121, and O145) serotypes, the antimicrobial resistance, virulence factors, and genomic structure of E. coli O177 are poorly understood. Therefore, in this article, we present the whole genome sequence data of two aEPEC E. coli O177 isolates (E. coli O177_CF-154-A and E. coli O177_CF-335-B) generated using Illumina MiSeq platform. The raw data were generated, cleaned, and assembled using Trimmomatic and SPAdes. Genome data analysis yielded 5,112,402 and 5,460,435 bp, comprising contigs 101 and 191 with GC contents of 50.7% and 50.5% for E. coli O177_CF-154-A and E. coli O177_CF-335-B, respectively. Prokaryotic Genome Annotation Pipeline (PGAP) and Rapid Annotation using Subsystem Technology (RAST) showed that the complete genome of E. coli O177_CF-154-A contained 5040 coding sequences (CDS), 5146 genes, 4896 proteins, 90 RNAs, and 78 tRNA while that of E. coli O177_CF-335-B contained 5463 CDS, 5570 genes, 5230 proteins, 92 RNAs, and 80 tRNA for. A total of 426 and 425 subsystem features with 5190 and 5662 CDS were obtained for E. coli O177_CF-154-A and E. coli O177_CF-335-B, respectively. Several genes encoding virulence and antimicrobial resistance were identified in both genomes. Complete genome sequence data of both isolates have been deposited in the National Center for Biotechnology Information (NCBI), GenBank: accession numbers, VMKH00000000 (E. coli O177_CF-154-A) and VMKG00000000 (E. coli O177_CF-335-B). This data can be used as a reference for determining the virulence and antimicrobial resistance in E. coli O177 isolates from different sample sources.

Keywords: Escherichia coli O177; Genome annotation; Genomic data; Virulence and Antimicrobial resistance genes; Whole genome sequence.