Nuclei Enrichment from Sugarcane Stems for Proteomics Analyses

Fernanda Salvato; Raquel Figueiredo; Paulo Mazzafera

doi:10.1007/978-1-0716-2185-1_7

Nuclei Enrichment from Sugarcane Stems for Proteomics Analyses

Methods Mol Biol. 2022:2469:79-87. doi: 10.1007/978-1-0716-2185-1_7.

Authors

Fernanda Salvato¹, Raquel Figueiredo^{2

3}, Paulo Mazzafera^{4

5}

Affiliations

¹ Plant and Microbial Biology Department, North Carolina State University, Raleigh, NC, USA.
² Department of Plant Biology, Institute of Biology, State University of Campinas, Campinas, Brazil.
³ Department of Biology, Faculty of Sciences and LAQV Requimte, Sustainable Chemistry, University of Porto, Porto, Portugal.
⁴ Department of Plant Biology, Institute of Biology, State University of Campinas, Campinas, Brazil. pmazza@unicamp.br.
⁵ Department of Crop Science, College of Agriculture Luiz de Queiroz, University of São Paulo, Piracicaba, Brazil. pmazza@unicamp.br.

PMID: 35508831
DOI: 10.1007/978-1-0716-2185-1_7

Abstract

Nuclei enrichment procedures enable a large variety of investigations. These studies include structural characterization of nuclear proteins, identification of posttranslational modifications, and regulation of stress or development-related gene expression. Successful enrichment of nuclei samples from plant tissues is crucial for a comprehensive analysis of the plant nuclear proteome. Here, we describe a method for nuclei enrichment from sugarcane stems and its assessment by western blot.

Keywords: Nuclei enrichment; Proteins; Stem; Sugarcane; Western blot.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Nucleus / metabolism
Edible Grain / chemistry
Plant Proteins / metabolism
Proteome / metabolism
Proteomics / methods
Saccharum* / genetics

Substances

Plant Proteins
Proteome