A panoptic review of techniques for finfish disease diagnosis: The status quo and future perspectives

Abstract

Disease outbreaks caused by bacterial and viral pathogens is a major impediment to the sustainable growth of aquaculture. Rapid and accurate diagnosis of pathogens is crucial for the successful maintenance of fish health and productivity in aquaculture. This review manuscript provides a brief description of conventional disease diagnosis techniques and a detailed description of immunological techniques such as ELISA, immunofluorescence, immunohistochemistry and lateral flow immunoassay. Specific emphasis has been given to detail the molecular techniques, such as PCR and its variants, including the novel isothermal amplification techniques like LAMP and RPA, that can cater to the need of rapid and sensitive point-of-care diagnostics. Hybridization-based methods and molecular typing methods have also been discussed as they find specific applications in diagnostics. The potential of novel techniques such as MALDI-TOF-MS, flow cytometry, and nanotechnology-based methods have also been outlined as they are likely to revolutionise disease diagnosis in the future. This manuscript provides an update on the principle, strengths, weaknesses, applications and variations of each technique, so as to eliminate the qualms for the adoption of these techniques in aquaculture diagnostics.

Keywords: Disease diagnosis; Immunological techniques; Isothermal amplification; Molecular typing; PCR.