The Role of Long Non-Coding RNA CCAT1 and SNHG14 in Activation of Some Protein-Coding Genes Associated with the Development of Ovarian Cancer

O I Brovkina; I V Pronina; A M Burdennyy; L A Uroshlev; E A Filippova; M V Fridman; T P Kazubskaya; K I Zhordania; V I Loginov; N E Kushlinskii; E A Braga

doi:10.1007/s10517-022-05473-8

The Role of Long Non-Coding RNA CCAT1 and SNHG14 in Activation of Some Protein-Coding Genes Associated with the Development of Ovarian Cancer

Bull Exp Biol Med. 2022 Apr;172(6):760-764. doi: 10.1007/s10517-022-05473-8. Epub 2022 May 2.

Authors

O I Brovkina^{1

2}, I V Pronina³, A M Burdennyy³, L A Uroshlev^{3

4}, E A Filippova³, M V Fridman⁴, T P Kazubskaya⁵, K I Zhordania⁵, V I Loginov³, N E Kushlinskii⁵, E A Braga³

Affiliations

¹ Research Institute of General Pathology and Pathophysiology, Moscow, Russia. brov.olia@gmail.com.
² Federal Research Clinical Center of Specialized Types of Medical Care and Medical Technologies, Federal Medical-Biological Agency of Russia, Moscow, Russia. brov.olia@gmail.com.
³ Research Institute of General Pathology and Pathophysiology, Moscow, Russia.
⁴ N. I. Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, Russia.
⁵ N. N. Blokhin National Medical Research Center of Oncology, Ministry of Health of the Russian Federation, Moscow, Russia.

PMID: 35501644
DOI: 10.1007/s10517-022-05473-8

Abstract

Late diagnosis of ovarian cancer is one of the most important problems in its treatment. Long non-coding RNA (lncRNA) are a poorly studied, but promising type of diagnostic biomarkers. We studied the lncRNA interactome to identify biomarkers with potential significance for molecular diagnostics of ovarian cancer. By screening the TCGA database, we identified differentially expressed lncRNA CCAT1 and SNHG14. Based on the indices of complementarity of CCAT1 and SNHG14 to the mRNA sequences, we selected 5 protein-coding genes MAPK1, c-MET, TGFB2, SNAIL1, and WNT4 associated with the epithelial-mesenchymal transition. Real-time PCR on 54 ovarian cancer samples confirmed the high expression levels of CCAT1 and SNHG14 (logFC>1.5, p<0.05). A positive correlation between the expression levels of two lncRNA and mRNA of 5 genes in 6 pairs was established. The activating effect of CCAT1 and SNHG14 on the expression of these genes can be mediated by miR-203 and miR-124.

Keywords: biomarkers; epithelial-mesenchymal transition; long non-coding RNA; microRNA; ovarian cancer.

MeSH terms

Carcinoma, Ovarian Epithelial / genetics
Carcinoma, Ovarian Epithelial / metabolism
Cell Proliferation / genetics
Female
Humans
MicroRNAs* / genetics
MicroRNAs* / metabolism
Ovarian Neoplasms* / genetics
Ovarian Neoplasms* / metabolism
RNA, Long Noncoding* / genetics
RNA, Long Noncoding* / metabolism
RNA, Messenger / genetics

Substances

MIRN203 microRNA, human
MicroRNAs
RNA, Long Noncoding
RNA, Messenger
long non-coding RNA CCAT2, human