Mouse lung mechanical properties under varying inflation volumes and cycling frequencies

K A M Quiros; T M Nelson; S Sattari; C A Mariano; A Ulu; E C Dominguez; T M Nordgren; M Eskandari

doi:10.1038/s41598-022-10417-3

Mouse lung mechanical properties under varying inflation volumes and cycling frequencies

Sci Rep. 2022 May 2;12(1):7094. doi: 10.1038/s41598-022-10417-3.

Authors

K A M Quiros¹, T M Nelson¹, S Sattari¹, C A Mariano¹, A Ulu^{2

3}, E C Dominguez^{2

3}, T M Nordgren^{2

3

4}, M Eskandari^{5

6

7}

Affiliations

¹ Department of Mechanical Engineering, University of California, Riverside, CA, USA.
² BREATHE Center, School of Medicine, University of California, Riverside, CA, USA.
³ Division of Biomedical Sciences, School of Medicine, University of California Riverside, Riverside, CA, USA.
⁴ Department of Environmental and Radiological Health Sciences, Colorado State University, Fort Collins, CO, USA.
⁵ Department of Mechanical Engineering, University of California, Riverside, CA, USA. mona.eskandari@ucr.edu.
⁶ BREATHE Center, School of Medicine, University of California, Riverside, CA, USA. mona.eskandari@ucr.edu.
⁷ Department of Bioengineering, University of California, Riverside, CA, USA. mona.eskandari@ucr.edu.

Abstract

Respiratory pathologies alter the structure of the lung and impact its mechanics. Mice are widely used in the study of lung pathologies, but there is a lack of fundamental mechanical measurements assessing the interdependent effect of varying inflation volumes and cycling frequency. In this study, the mechanical properties of five male C57BL/6J mice (29-33 weeks of age) lungs were evaluated ex vivo using our custom-designed electromechanical, continuous measure ventilation apparatus. We comprehensively quantify and analyze the effect of loading volumes (0.3, 0.5, 0.7, 0.9 ml) and breathing rates (5, 10, 20 breaths per minute) on pulmonary inflation and deflation mechanical properties. We report means of static compliance between 5.4-16.1 µl/cmH₂O, deflation compliance of 5.3-22.2 µl/cmH₂O, percent relaxation of 21.7-39.1%, hysteresis of 1.11-7.6 ml•cmH₂O, and energy loss of 39-58% for the range of four volumes and three rates tested, along with additional measures. We conclude that inflation volume was found to significantly affect hysteresis, static compliance, starting compliance, top compliance, deflation compliance, and percent relaxation, and cycling rate was found to affect only hysteresis, energy loss, percent relaxation, static compliance and deflation compliance.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Insufflation*
Lung Compliance
Lung Volume Measurements
Lung*
Male
Mice
Mice, Inbred C57BL