Development and single laboratory validation of a targeted liquid chromatography-triple quadrupole mass spectrometry-based method for the determination of insulin like growth factor-1 in different types of milk samples

Giulia Remaggi; Roberta Saleri; Melania Andrani; Francesca Satolli; Eleonora Rodighiero; Lisa Elviri

doi:10.1016/j.fochx.2022.100271

Development and single laboratory validation of a targeted liquid chromatography-triple quadrupole mass spectrometry-based method for the determination of insulin like growth factor-1 in different types of milk samples

Food Chem X. 2022 Feb 24:13:100271. doi: 10.1016/j.fochx.2022.100271. eCollection 2022 Mar 30.

Authors

Giulia Remaggi¹, Roberta Saleri², Melania Andrani², Francesca Satolli², Eleonora Rodighiero³, Lisa Elviri¹

Affiliations

¹ Department of Food and Drug Science, University of Parma, Parco Area delle Scienze 27/A, 43124 Parma, Italy.
² Department of Veterinary Science, University of Parma, Via del Taglio 10, 43100 Parma, Italy.
³ Section of Dermatology, Department of Medicine and Surgery, University of Parma, Via Gramsci, 43100 Parma, Italy.

Abstract

A simple and reliable targeted liquid chromatography-electrospray-tandem mass spectrometry (LC-MS/MS) method was developed and validated through the selection of two biomarker peptides for the identification and determination of bovine insulin like growth factor-1 (IGF-1) in milk samples. Two urea-based sample extraction procedures were tested. The validation results provided detection limits at the 1-5 ng IGF-1/mL level as a function of the milk matrix, precision ranged from 3 to 8% and the method accuracy in the different milk matrices was assured. Finally, IGF-1 was measured in milk samples obtained by treatment with eleven different technological processes: IGF-1 concentrations were spread over a wide range from 11.2 ± 0.3 ng/mL to 346 ± 8 ng/mL with a median of 57.0 ± 0.2 ng/mL. The highest amount of IGF-1 was found in fresh whole milk samples and no significant correlation was found between the total milk protein content and the IGF-1 concentration level.

Keywords: Cow milk samples; Insulin like growth factor-1 distribution; Method validation; Urea-based extraction.