A comparison study between ultrasound-assisted and enzyme-assisted extraction of anthocyanins from blackcurrant (Ribes nigrum L.)

María José Aliaño González; Ceferino Carrera; Gerardo F Barbero; Miguel Palma

doi:10.1016/j.fochx.2021.100192

A comparison study between ultrasound-assisted and enzyme-assisted extraction of anthocyanins from blackcurrant (Ribes nigrum L.)

Food Chem X. 2021 Dec 21:13:100192. doi: 10.1016/j.fochx.2021.100192. eCollection 2022 Mar 30.

Authors

María José Aliaño González¹, Ceferino Carrera¹, Gerardo F Barbero¹, Miguel Palma¹

Affiliation

¹ Department of Analytical Chemistry, Faculty of Sciences, University of Cadiz, Agrifood Campus of International Excellence (ceiA3), IVAGRO, 11510 Puerto Real, Cadiz, Spain.

Abstract

Blackcurrant (Ribes nigrum L.) is a fruit rich in vitamins, fatty acids, minerals, essential oils and phenolic compounds, including anthocyanins. In the present work, two anthocyanin extraction methods from blackcurrant samples based on Ultrasound-Assisted Extraction (UAE) and Enzyme-Assisted Extraction (EAE) have been developed. A Plackett-Burman design with seven variables has been preliminary used for both UAE and EAE in order to determine the most influential variables in each methodology. After that, a Box-Behnken design was employed to optimize the extraction methods. The composition of the extraction solvent (% EtOH in water) has been the most influential variable for both UAE and EAE. The optimal extraction times have been 5 min for UAE and 10 min for EAE. No differences have been observed in anthocyanin extraction with both methodologies. Both methods have been applied to blackcurrant-derived products and proven their suitability for quality control analysis.

Keywords: Anthocyanins; Blackcurrant; Box-Behnken; Comparison; Delphinidin 3–O–glucoside (PubChem CID: 443650); Enzyme–assisted extraction; Plackett–Burman; Ribes nigrum; Ultrasound–assisted extraction; cyanidin 3–O–glucoside (PubChem CID: 44256715); cyanidin 3–O–rutinoside (PubChem CID: 441674); delphinidin 3–O–rutinoside (PubChem CID: 192918); pelargonidin 3–O–rutinoside (PubChem CID: 443917); peonidin 3–O–rutinoside (PubChem CID: 44256842); petunidin 3–O–rutinoside (PubChem CID: 44256957).