Chitosan is a biostimulator that has a great effect either on plant physiology, productivity, or fruit quality. However, the metabolic mechanism regulated by chitosan still remains unknown. Untargeted metabolomics analysis, using LC-MS/MS mass spectrometry, was used to investigate fruit quality markers. Thus, this study was focused on the identification of untargeted metabolites of tomato fruits produced under the application of five doses of chitosan at different concentrations (0, 0.25, 0.50, 0.75, and 1 mg ml-1) that was extracted from Parapenaeus longirostris shrimp shells. The identification was carried out using two ion modes (ESI-/ESI+), a web application "Metfamily" to analyze signals, and reference libraries. The analysis of data using partial least squares discriminant analysis (PLS-DA) and hierarchical cluster analysis (HCA) showed that chitosan application, especially 0.75 mg ml-1, had a clear and remarkable effect regarding the number of metabolite families identified in both ion modes. This treatment has increased the relative abundance of many metabolites that belong to anthocyanins decorated with sugars, terpenoids, phenylpropanoids, acylsugars, glucosinolates, folates, galactolipids, fatty acids, and phospholipids. Thus, these results showed that chitosan application increased the quality of tomato fruits due to its involvement in the regulation of many metabolic pathways that might be responsible for enhancing the nutritional characteristics as well as the defense of fruits.
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