Electrochemical immunosensor based on AuBP@Pt nanostructure and AuPd-PDA nanozyme for ultrasensitive detection of APOE4

Yibiao Liu; Guangli He; Huili Liu; Hang Yin; Fengli Gao; Jian Chen; Shouren Zhang; Baocheng Yang

doi:10.1039/d0ra00298d

Electrochemical immunosensor based on AuBP@Pt nanostructure and AuPd-PDA nanozyme for ultrasensitive detection of APOE4

RSC Adv. 2020 Feb 24;10(13):7912-7917. doi: 10.1039/d0ra00298d. eCollection 2020 Feb 18.

Authors

Yibiao Liu¹, Guangli He¹, Huili Liu¹, Hang Yin¹, Fengli Gao¹, Jian Chen¹, Shouren Zhang¹, Baocheng Yang¹

Affiliation

¹ Department of Henan Key Laboratory of Nanocomposites and Applications, Institute of Nanostructured Functional Materials, Huanghe Science and Technology College Zhengzhou 450006 China liuyibiao12345@126.com jianchen@infm.hhstu.edu.cn.

Abstract

An ultrasensitive sandwich-type electrochemical immunosensor based on AuBP@Pt nanostructures and AuPd-PDA nanozyme was developed for the detection of apolipoprotein E4 (APOE4) which was an important risk factor for Alzheimer's disease (AD). In this work, gold nanobipyramid coated Pt (AuBP@Pt) nanostructures were prepared and applied to electrochemical immunosensors as a substrate material. AuBP@Pt nanostructures have advantages of electrical conductivity and large electroactive area, which could greatly increase electron transfer rate. In previous work, we designed AuPd alloy modified polydopamine (AuPd-PDA) nanozyme which catalyzed the decomposition of hydrogen peroxide (H₂O₂). AuPd-PDA nanozyme was used to label detection antibody due to excellent catalytic capability and stability in this new paper. And the concentration of APOE4 could be detected quantitatively by variation for transient current. As a result, the electrochemical immunosensor based on AuBP@Pt and AuPd-PDA exhibited a wide linear range from 0.05 to 2000 ng mL^-1 and low detection limit of 15.4 pg mL^-1 (S/N = 3). Furthermore, the designed biosensor displayed good selectivity in phosphate buffer saline (PBS) buffer solution or commercial goat serum, which provided a promising tool for early diagnosis of AD.